Virus Genes

, Volume 15, Issue 2, pp 119–121 | Cite as

Sequencing of a 5.5-kb DNA Fragment and Identification of a Gene Coding for a Subunit of the Helicase/Primase Complex of Avian Laryngotracheitis virus (ILTV)

  • Qinyun Huang
  • Yahya Mat-arip
  • Peixuan Guo
Article

Abstract

The nucleotide sequence of a 5,520-bp EcoRI restriction fragment of avian infectious laryngotracheitis (ILTV) DNA was reported and submitted to GeneBank with an accession number of AF001078. Computer prediction revealed one large potential open reading frame (ORF) with sequence similar to one subunit of the DNA helicase-primase complex of α-herpesviruses. The DNA helicase/primase complex of HSV-1 consists of three sub-units with molecular weights of 12,000, 97,000 and 70,000, encoded by genes UL52, UL5 and UL8, respectively. This enzyme complex is essential for herpesvirus DNA replication. The UL52 and UL5-equivalent genes of ILTV have been reported previously (Fuchs, W. and Mettenleiter, T.C., J Gen Virol, 1996, 77: 2221–2229; Johnson, M.A. et. al., Arch Virol, 1995, 14: 623–634). Amino acid sequence comparison and homology search revealed that this ORF shares sequence similarity to the UL8-equivalent gene of α-herpesviruses, that is, the ORF 52 of vericura-zoster virus (VZV), the ORF 54 of equine herpesvirus type-1 (EHV-1), as well as the equivalent gene of bovine herpesvirus type 1(BHV-1) and canine herpesvirus (Vlcek, C. et al., Virology, 1995, 210: 100–108; Remond, M. et al., J Gen Virol, 1996, 77: 37–48).

DNA helicase primase complex herpesvirus DNA UL8 ILTV DNA replicase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Roizman B., Carmichael L.E., Deinhardt F., de-The G., Nahmias A.J., Plowright W., Rapp F., Sheldrick P., Takahashi M., and Wolf K., Intervirology 16, 201–217, 1981.PubMedGoogle Scholar
  2. 2.
    Griffin A.M., J Gen Virol 72, 393–398, 1991.PubMedGoogle Scholar
  3. 3.
    Johnson M.A., and Tyack S., Vet Microbiol 46, 221–231, 1995.PubMedCrossRefGoogle Scholar
  4. 4.
    Johnson M.A., Prideaux C.T., Kongsuwan K., Tyack S.G., and Sheppard M., Arch Virol 140, 623–634, 1995.PubMedCrossRefGoogle Scholar
  5. 5.
    Griffin A.M., and Boursnell M.E.G., J Gen Virol 71, 841–850, 1990.PubMedGoogle Scholar
  6. 6.
    Keeler C.L., Kingsley D.H., and Adams-Burton C.R., Avian Dis 35, 920–929, 1991.PubMedCrossRefGoogle Scholar
  7. 7.
    Guo P., Scholz E., Maloney B., and Welniak E., Virology 202, 771–781, 1994.PubMedCrossRefGoogle Scholar
  8. 8.
    Kongsuwan K., Prideaux C.T., Johnson M.A., Sheppard M., and Fahey K.J., Virology 184, 404–410, 1991.PubMedGoogle Scholar
  9. 9.
    Griffin A.M., Nucleic Acids Res 18, 3664, 1990.PubMedGoogle Scholar
  10. 10.
    Fuchs W. and Mettenleiter T.C., J Gen Virol 77, 2221–2229, 1996.PubMedGoogle Scholar
  11. 11.
    Johnson M.A., Tyack S.G., Prideaux C.T., Kongsuwan K., and Sheppard M., DNA Seq 5, 191–194, 1995.PubMedGoogle Scholar
  12. 12.
    Kingsley D.H., Hazel J.W., and Keeler C.L. Jr., Virology 203, 336–343, 1994.PubMedCrossRefGoogle Scholar
  13. 13.
    Johnson M.A., Tyack S.G., Prideaux C., Kongsuwan K., and Sheppard M., Virus Res 35, 193–204, 1995.PubMedCrossRefGoogle Scholar
  14. 14.
    Sheppard M. and York J.J., Acta Virol 34, 443–448, 1990.PubMedGoogle Scholar
  15. 15.
    Kongsuwan K., Johnson M.A., Prideaux C.T., and Sheppard M., Virus Res 29, 125–140, 1993.PubMedCrossRefGoogle Scholar
  16. 16.
    Scholz E., Welniak E., Nyholm T., and Guo P., J Virol Meth 43, 273–286, 1993.CrossRefGoogle Scholar
  17. 17.
    Schnitzlein W.M., Radzevicius J., and Tripathy D.N., Avian Dis 38, 211–217, 1994.PubMedCrossRefGoogle Scholar
  18. 18.
    Guo P., Scholz E., Turek J., Nordgren R., and Maloney B., Am J Vet Res 54, 2031–2039, 1993.PubMedGoogle Scholar
  19. 19.
    Scholz E., Zhang C.L., and Guo P., J Virol Meth 45, 291–301, 1993.CrossRefGoogle Scholar
  20. 20.
    Scholz E., and Guo P., Construction of Recombinant Thymidine Kinase-Negative Infectious Laryngotracheitis Virus Proc 3rd Congress Europ Soc Vet Virol 379–384, 1995.Google Scholar
  21. 21.
    Crute J.J., Tsurumi T., Zhu L., Weller S.K., Olivo P.D., Challberg M.D., Mocarski E.S., and Lehman I.R., Proc Natl Acad Sci USA 86, 2186–2189, 1989.PubMedCrossRefGoogle Scholar
  22. 22.
    Klinedinst D.K., and Challberg M.D., J Virol 68, 3693–3701, 1994.PubMedGoogle Scholar
  23. 23.
    Devereaux J., Haeberli P., and Smithies O., Nucleic Acids Res 12, 387–395, 1984.Google Scholar
  24. 24.
    Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J., J Mol Biol 215, 403–410, 1990.PubMedCrossRefGoogle Scholar
  25. 25.
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perr L.J., Scott J.E., and Taylor P., J Gen Virol 69, 1531–1574, 1988.PubMedCrossRefGoogle Scholar
  26. 26.
    Vlcek C., Benes V., Lu Z., Kutish G.F., Paces V., Rock D., Letchworth G.J., and Schwyzer M., Virology 210, 100–108, 1995.PubMedCrossRefGoogle Scholar
  27. 27.
    Remond M., Sheldrick P., Lebreton F., Nardeux P., and Foulon T., J Gen Virol 77 (Pt 1), 37–48, 1996.PubMedGoogle Scholar
  28. 28.
    Glynias, M.J., GeneWorks, Mountain View, California, Intelligenetics, Inc, 1991.Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Qinyun Huang
    • 1
  • Yahya Mat-arip
    • 1
  • Peixuan Guo
    • 1
  1. 1.Department of Veterinary Pathobiology, School of Veterinary MedicinePurdue UniversityWest LafayetteUSA

Personalised recommendations