Fish Physiology and Biochemistry

, Volume 22, Issue 2, pp 125–133 | Cite as

Effect of a prolactin pharmacological stimulant (sulpiride) and suppressant (bromocriptine) on heat shock protein 70 expression in silver sea bream, Sparus sarba

  • E.E. Deane
  • S.P. Kelly
  • I.N.K. Chow
  • N.Y.S. Woo

Abstract

Heat shock protein 70 (HSP70) expression was assessed in hepatic tissue of silver sea bream, Sparus sarba. Using a combination of reverse transcription and PCR we have cloned a 794 base pair HSP70 cDNA fragment. This clone hybridized to a 2.3kb HSP70 mRNA transcript which was elevated 2.5 fold after acute heat shock exposure. Cold shock however, had no effect on HSP70 expression. In order to study the effect of prolactin on silver sea bream hepatic HSP70, groups of fish received either ovine prolactin (oPRL), sulpiride or bromocriptine, daily, over a seven day period. Treatment with oPRL and sulpiride resulted in a reduction in the relative abundance of hepatic HSP70 mRNA by 48 and 52%, respectively. Also hepatic HSP70 levels were reduced by 69 and 46% (as determined by immunoblotting) after oPRL and sulpiride administration, respectively. The administration of bromocriptine significantly increased both HSP70 mRNA and protein levels by 197 and 240%, respectively. The data from this study provides strong support for the importance of prolactin in regulating HSP70 expression in silver sea bream.

hormone HSP70 mRNA expression protein expression seabream teleost fish 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • E.E. Deane
    • 1
  • S.P. Kelly
    • 1
  • I.N.K. Chow
    • 1
  • N.Y.S. Woo
    • 1
  1. 1.Department of BiologyThe Chinese University of Hong KongShatin, N. T.Hong Kong SAR, China

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