Fish Physiology and Biochemistry

, Volume 16, Issue 6, pp 479–485

Dietary spermine supplementation induces intestinal maturation in sea bass (Dicentrarchus labrax) larvae

  • A. Péres
  • C.L. Cahu
  • J.L. Zambonino Infante


Sea bass (Dicentrarchus labrax) larvae were fed microparticulated compound diet containing 0 (FP0), 0.10 (FP10) and 0.33% (FP33) of a polyamine, spermine, from day 20 to day 38. LP group was fed live prey. This group exhibited the highest growth and survival. The addition of spermine did not lead to growth enhancement. A 33% survival improvement was obtained in FP33 group compared to FP0 group. The spermine addition affected the activity of pancreatic enzymes, trypsin, chymotrypsin and amylase, during larvae development. This non specific effect suggested that the action of spermine would be mediated by hormones. In the intestine, the FP33 group exhibited from day 31 higher activities of brush border membrane enzymes (leucine aminopeptidase and alkaline phosphatase) and lower level in a cytosolic enzyme (leucine-alanine peptidase) compared to FP10 and FP0 group. The diet containing the highest spermine level induced an enzymatic profile similar to that obtained in LP group and characteristic of a mature enterocyte. The initiation of enterocyte maturation at a proper development stage was associated to the survival improvement observed in FP33 group.

polyamine spermine sea bass larvae intestinal maturation pancreatic enzymes intestinal enzymes 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References cited

  1. Bardócz, S., Grant, G., Brown, D.S. Ralph, A. and Pusztai, A. 1993. Polyamines in food-implications for growth and health. J. Nutr. Biochem. 4: 66–71.Google Scholar
  2. Bardócz, S. 1995. Polyamines in food and their consequences for food quality and human health. Trends Food Sci. Technol. 6: 341–346.Google Scholar
  3. Bessey, O.A., Lowry, O.H. and Brock, M.J. 1946. Rapid coloric method for determination of alkaline phosphatase in five cubic millimeters of serum. J. Biol. Chem. 164: 321–329.Google Scholar
  4. Bidlingmeyer, B.A., Cohen, S.A. and Tarvin, T.L. 1984. Rapid analysis of amino acids using pre-column derivatization. J. Chromatogr. 336: 93–104.PubMedGoogle Scholar
  5. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.PubMedGoogle Scholar
  6. Buddington, R.K. 1985. Digestive secretions of lake sturgeon, Acipenser fulvescens, during early development. J. Fish Biol. 26: 715–723.Google Scholar
  7. Buts, J.P., De Keyser, N., Kolanowski, J., Sokal, E. and Van Hoof, F. 1993. Maturation of villus and crypt cell functions in rat small intestine. Role of dietary polyamines. Dig. Dis. Sci. 38: 1091–1098.PubMedGoogle Scholar
  8. Cahu, C.L. and Zambonino Infante, J.L. 1994. Early weaning of sea bass (Dicentrarchus labrax) larvae with a compound diet: effect on digestive enzymes. Comp. Biochem. Physiol. 109A: 213–222.Google Scholar
  9. Cahu, C.L. and Zambonino Infante, J.L. 1995. Maturation of the pancreatic and intestinal digestive function in sea bass (Dicentrarchus labrax): effect of weaning with different protein sources. Fish Physiol. Biochem. 14: 431–437.Google Scholar
  10. Dufour, C., Dandrifosse, G., Forget, P., Vermess, F., Romain, N. and Lepoint, P. 1988. Spermine and spermidine induce intestinal maturation in the rat. Gastroenterology 95: 112–116.PubMedGoogle Scholar
  11. Crane, R.K., Boge, G. and Rigal, A. 1979. Isolation of brush border membranes in vesicular form from the intestinal spiral valve of the small dogfish (Scyliorhinus canicula). Biochim. Biophys. Acta 554: 264–267.PubMedGoogle Scholar
  12. Fioramonti, J., Fargeas, M.J., Bertrand, V., Pradayrol, L. and Buéno, L. 1994. Induction of postprandial intestinal motility and release of cholecystokinin by polyamines in rats. Am. J. Physiol. 267: G960–G965.PubMedGoogle Scholar
  13. Gawlicka, A., Teh, S.J., Hung, S.S.O., Hinton, D.E. and de la Noüe, J. 1995. Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny. Fish Physiol. Biochem. 14: 357–371.Google Scholar
  14. Henning, S.J. 1987. Functional development of the gastrointestinal tract. In Physiology of the Gastrointestinal Tract. pp. 285–300. 2nd edition. Edited by L.R. Johnson. Raven Press, New York.Google Scholar
  15. Holm, H., Hanssen, L.E., Krogdahl, A. and Florholmen, J. 1988. High and low inhibitor soybean meals affect human duodenal proteinase activity differently: in vivo comparison with bo-vine serum albumin. J. Nutr. 118: 515–520.PubMedGoogle Scholar
  16. Kaouass, M., Sulon, J., Deloyer, P. and Dandrifosse, G. 1994. Spermine-induced precocious intestinal maturation in suckling rats: possible involvement of glucocorticoids. J. Endocrinol. 141: 279–283.PubMedGoogle Scholar
  17. Kjøfrsvik, E. and Reiersen, A.L. 1992. Histomorphology of the early yolk-sac larvae of the Atlantic halibut (Hippoglossus hippoglossus L.)–an indication of the timing of functionality. J. Fish Biol. 41: 1–19.Google Scholar
  18. Lhoste, E.F., Fiszlewicz, M., Gueugneau, A.M., Tranchant, T. and Corring, T. 1994. Early adaptation of pancreas to a protein-enriched diet: role of cholescystokinin and gastrin-releasing peptide. Pancreas 9: 624–632.PubMedGoogle Scholar
  19. Maroux, S., Louvard, D. and Baratti, J. 1973. The aminopeptidase from hog-intestinal brush-border. Biochim. Biophys. Acta 321: 282–295.PubMedGoogle Scholar
  20. Métais, P. and Bieth, J. 1968. Détermination de l'á-amylase par une microtechnique. Ann. Biol. Clin. 26: 133–142.Google Scholar
  21. Nicholson, J.A. and Kim, Y.S. 1975. A one-step L-amino acid oxidase assay for intestinal peptide hydrolase activity. Anal. Biochem. 63: 110–117.Google Scholar
  22. Person-Le Ruyet, J., Alexandre, J.C., Thébaud, L. and Mugnier, C. 1993. Marine fish larvae feeding: formulated diets or live Preys? J. World Aquac. Soc., 24: 211–224.Google Scholar
  23. Smith, T.K. 1990. Effect of dietary putrescine on whole body growth and polyamine metabolism. P. S. E. B. M. 194: 332–336.Google Scholar
  24. Sousadias, M.G. and Smith, T.K. 1995. Toxicity and growth-promoting of spermine when fed to chicks. J. Anim. Sci. 73: 2375–2381.PubMedGoogle Scholar
  25. Tabor, C.W. and Tabor, H. 1984. Polyamines. Ann. Rev. Biochem. 53: 749–790.PubMedGoogle Scholar
  26. Vanderhoof, J.A. 1993. Regulatory peptides and intestinal growth. Gastroenterology 104: 1205–1208.PubMedGoogle Scholar
  27. Wéry, I., Deloyer, P. and Dandrifosse, G. 1996. Effects of single dose of orally-administrated spermine on the intestinal development of unweaned rats. Arch. Physiol. Biochim. 104:163–172.Google Scholar
  28. Worthington, T.M. 1982. Enzymes and Related Biochemicals. Biochemical Products Division. Worthington Diagnostic System Inc., Freehold.Google Scholar
  29. Zambonino Infante, J.L. and Cahu, C.L. 1994. Development and response to a diet change of some digestive enzymes in sea bass (Dicentrarchus labrax) larvae. Fish Physiol. Biochem. 12: 399–408.Google Scholar
  30. Zambonino Infante, J.L., Cahu, C.L. and Péres, A. 1997. Partial substitution of native fish meal protein by di-and tripeptides in diet improves sea bass (Dicentrarchus labrax) larvae development. J. Nutr. (In press).Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • A. Péres
    • 1
  • C.L. Cahu
    • 1
  • J.L. Zambonino Infante
    • 1
  1. 1.Unité Mixte de Nutrition des Poissons IFREMER-INRAIFREMER Centre de BrestPlouzanéFrance

Personalised recommendations