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Neurochemical Research

, Volume 25, Issue 3, pp 385–387 | Cite as

The Na+-Ca2+ Exchange Inhibitor KB-R7943 Inhibits High K+-Induced Increases in Intracellular Ca2+ Concentration and [3H]Noradrenaline Release in the Human Neuroblastoma SH-SY5Y

  • Hiroaki Nakamura
  • Yasushi Kawasaki
  • Naohisa Arakawa
  • Makio Saeki
  • Sadaaki Maeda
  • Yutaka Koyama
  • Akemichi Baba
  • Toshio Matsuda
Article

Abstract

The effects of the Na+-Ca2+ exchange inhibitor 2-[2-[4-(4-nitrobenzyloxy)phenyl]ethyl]isothiourea methanesulfonate (KB-R7943) on depolarization-induced Ca2+ signal and [3H]noradrenaline release were examined in SH-SY5Y cells. KB-R7943 at 10 μM significantly inhibited high K+-induced increase in intracellular Ca2+ concentration. KB-R7943 also inhibited high K+-evoked release of [3H]noradrenaline from the cells. These findings suggest that the Na+-Ca2+ exchanger in the reverse mode is involved at least partly in depolarization-induced transmitter release.

Na+-Ca2+ exchange KB-R7943 inhibitor noradrenaline release 

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Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Hiroaki Nakamura
    • 1
  • Yasushi Kawasaki
    • 2
  • Naohisa Arakawa
    • 1
  • Makio Saeki
    • 3
  • Sadaaki Maeda
    • 3
  • Yutaka Koyama
    • 1
  • Akemichi Baba
    • 1
  • Toshio Matsuda
    • 4
  1. 1.Laboratory of Molecular NeuropharmacologyJapan
  2. 2.Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical SciencesOsaka UniversityOsakaJapan
  3. 3.Department of Pharmacology, Faculty of DentistryOsaka UniversityOsakaJapan
  4. 4.aboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical SciencesOsaka UniversityOsakaJapan

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