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Plant Molecular Biology Reporter

, Volume 16, Issue 3, pp 257–262 | Cite as

Binary Vectors for Sense and Antisense Expression of Arabidopsis ESTs

  • Joshua Mylne
  • José Ramón Botella
Article

Abstract

Our laboratory is interested in devising methods to identify functions for the vast numbers of arabidopsis genes now available. For this purpose, we have constructed a set of binary vectors that will allow the quick production of transgenic arabidopsis plants containing either sense or antisense copies of EST clones obtained from the PRL2 library. These vectors are based on the pSLJ series containing the bialophos resistance (BAR) gene that confers resistance to the herbicide BASTA. In addition, our vectors contain a 35S CaMV promoter-polylinker-nos terminator cassette that allows the direct cloning of arabidopsis ESTs in either antisense (pAOV and pAOV2) or sense (pSOV and pSOV2) orientation. We also describe the construction of two additional vectors conferring BASTA resistance and containing the pBluescript polylinker in both orientations inserted between the 35S CaMV promoter and nos terminator (pKMB and pSMB).

antisense constructs arabidopsis binary vector EST herbicide resistance transgenic sense constructs 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Joshua Mylne
    • 1
  • José Ramón Botella
    • 1
  1. 1.Plant Genetic Engineering Laboratory, Department of BotanyThe University of QueenslandBrisbaneAustralia

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