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Scinderin, a Ca2+-Dependent Actin Filament Severing Protein that Controls Cortical Actin Network Dynamics During Secretion

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Abstract

Secretory vesicles are localized in specific compartments within neurosecretory cells. These are different pools in which vesicles are in various states of releasability. The transit of vesicles between compartments is controlled and regulated by Ca2+, scinderin and the cortical F-actin network. Cortical F-actin disassembly is produced by the filament severing activity of scinderin. This Ca2+-dependent activity of scinderin together with its Ca2+-independent actin nucleating activity, control cortical F-actin dynamics during the secretory cycle. A good understanding of the interaction of actin with scinderin and of the role of this protein in secretion has been provided by the analysis of the molecular structure of scinderin together with the use of recombinant proteins corresponding to its different domains.

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Authors and Affiliations

  1. Secretory Process Research Program, Department of Cellular & Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada, K1H 8M5

    J-M. Trifaró & S. D. Rosé

  2. 9610 Medical Center Drive, Suite 300, Rockville, MD, 20850

    M. G. Marcu (Dr.)

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Trifaró, JM., Rosé, S.D. & Marcu, M.G. Scinderin, a Ca2+-Dependent Actin Filament Severing Protein that Controls Cortical Actin Network Dynamics During Secretion. Neurochem Res 25, 133–144 (2000). https://doi.org/10.1023/A:1007503919265

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