Plant Molecular Biology Reporter

, Volume 16, Issue 2, pp 133–133 | Cite as

Isolation of DNA from the Highly Mucilagenous Succulent Plant shape Sedum telephium

  • Paul Barnwell
  • André N. Blanchard
  • John a. Bryant
  • Nicholas Smirnoff
  • A. Fred Weir
Article

Abstract

The C3-CAM intermediate plant Sedum telephium is very rich in mucilages (gelling polysaccharides) which present a major obstacle to the isolation of DNA. We have developed an extraction procedure that involves a step-wise increase in the concentration of CTAB (cetyl-trimethylammonium bromide) finally yielding a nucleic acid precipitate that is free from polysaccharides. DNA may be further purified by differential precipitation with LiCl and/or RNase digestion. The DNA has excellent spectral qualities, is readily accessible to restriction endonucleases and is usable as a PCR template.

CAM (Crassulacean acid metabolism) plant CTAB (cetyl-trimethylammonium bromide) DNA polysaccharide Sedum telephium 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Paul Barnwell
    • 1
  • André N. Blanchard
    • 1
  • John a. Bryant
    • 1
  • Nicholas Smirnoff
    • 1
  • A. Fred Weir
    • 2
  1. 1.School of Biological SciencesUniversity of ExeterExeterUK
  2. 2.Advanced Technologies (Cambridge) LtdCambridgeUK

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