Plant Molecular Biology Reporter

, Volume 16, Issue 2, pp 175–175

Efficient Amplification of Insert End Sequences from Bacterial Artificial Chromosome Clones by Thermal Asymmetric Interlaced PCR

  • Yao-Guang Liu
  • Ning Huang
Article

Abstract

TAIL-PCR is a powerful tool for the recovery of DNA fragments adjacent to known sequences. A protocol is presented for the amplification of insert end sequences from bacterial artificial chromosome clones using TAIL-PCR. The amplified products are suitable as probes for chromosome walking and genome mapping and as templates for direct sequencing. The protocol has been used in rice genome studies. Abbreviations: AD primer, arbitrary degenerate primer; BAC, bacterial artificial chromosome.

bacterial artificial chromosome chromosome walking insert end sequences 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Yao-Guang Liu
    • 1
  • Ning Huang
    • 2
  1. 1.Biotechnology CenterSouth China Agricultural UniversityGuangzhouChina
  2. 2.International Rice Research InstituteManilaPhilippines

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