Mycopathologia

, Volume 149, Issue 1, pp 5–12 | Cite as

The effect of the Fusarium metabolite beauvericin on electromechanical and -physiological properties in isolated smooth and heart muscle preparations of guinea pigs

  • R. Lemmens-Gruber
  • B. Rachoy
  • E. Steininger
  • K. Kouri
  • P. Saleh
  • R. Krska
  • R. Josephs
  • M. Lemmens
Article

Abstract

The electromechanical and -physiological effects of beauvericin were studied in isolated smooth and heart muscle preparations of the guinea pig. Beauvericin concentration-dependently decreased the force of contraction in precontracted (60 mM KCl) terminal ilea with an IC50 of 0.86 μM, and in electrically stimulated (1 Hz) papillary muscles with an IC50 of 18 μM. This negative inotropic effect in papillary muscles was antagonised in a non-competitive way by increased extracellular calcium concentrations. Spontaneous activity in right atria was affected at concentrations >10 μM beauvericin. The negative chronotropic effect was less pronounced than the negative inotropic effect. In action potentials of electrically driven (1 Hz) papillary muscles, 10 μM beauvericin significantly decreased membrane resting potential until unexcitability of the preparation occurred. Despite depolarisation of the membrane the maximum rate of rise of the action potential was not changed. The action potential duration was shortened, but the decrease was only significant at times to 20% and 50% repolarisation. These data, derived from the electrophysiological experiments, not only imply an effect on the calcium current as suggested by the effects on contractility, but also an interaction with the sodium inward and potassium outward currents.

action potential beauvericin force of contraction fusarium metabolite smooth muscle heart muscle 

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References

  1. 1.
    Vey A, Quiot J-M, Vago C. Mise en évidence et étude de l'action d'une mycotoxine, la beauvericine, sur des cellules d'insectes cultivées in vitro. CR Acad Sc Paris 1973; 276: 2489-2492.Google Scholar
  2. 2.
    Di Paola R, Nenna S, Fomelli F, Moretti A, Logrieco A, Caiaffa MF, Botallico A, Tursi A, Macchia L. Cytotoxicity of Beauvericin on human B-lymphocyte cell lines. Allergy and clinic Immun 1994; 2: 256 (Abstract).Google Scholar
  3. 3.
    Ojcius DM, Zychlinsky A, Zheng LM, Young JD. Ionophore-induced apoptosis: a role of DNA fragmentation and calcium fluxes. Exp Cell Res 1991; 197: 43-49.PubMedGoogle Scholar
  4. 4.
    Hamill RK, Higgens CE, Boaz HE, Gorman M. The structure of beauvericin: a new depsipeptide antibiotic toxic to Artemia salina. Tetrahed Lett 1969: 4255-4258.Google Scholar
  5. 5.
    Logrieco A, Moretti A, Altomare C, Bottalico A, Carbonell Torres E Occurrence and toxicity of Fusarium subglutinans from Peruvian maize. Mycopathologia 1993; 122: 185-190.CrossRefPubMedGoogle Scholar
  6. 6.
    Grove JF, Pople M. The insecticidal activity of beauvericin and the enniatin complex. Mycopathologia 1980; 70: 103-105.CrossRefGoogle Scholar
  7. 7.
    Gupta S, Krasnoff SB, Underwood NL, Renwick J AA, Roberts DW. Isolation of beauvericin as an insect toxin from Fusarium semitectum and Fusarium moniliforme var. subglutinans. Mycopathologia 1991; 115: 185-189.CrossRefPubMedGoogle Scholar
  8. 8.
    Tomoda H, Huang XH, Cao J, Nishida H, Nagao R, Okuda S, Tanaka H, Omura S, Arai H, Inoue K. Inhibition of acyl-CoA: cholesterol acyltransferase activity by cyclodepsipeptide antibiotics 1992; 45: 1626-1632.Google Scholar
  9. 9.
    Logrieco A, Moretti A, Castella G, Kostecki M, Golinski P, Ritieni A, Chelkowski J. Beauvericin production by Fusarium species. Appl Environ Microbiol 1998; 64: 3084-3088.PubMedGoogle Scholar
  10. 10.
    Shemyakin MM, Ovchinnikov YA, Ivanov VT, Antonov VK, Vinogradova EI, Shkrob AM, Malenkov GG, Evstratov A V, Laine IA, Melnik EI, Ryabova ID. Cyclodepsipeptides as chemical tools for studying ionic transport through membranes. J Membrane Biol 1969; 1: 402-430.CrossRefGoogle Scholar
  11. 11.
    Ivanov VT, Evstatov AV, Sumskaya LV, Melnik EI, Chumbridze TS, Portnova SL, Balashova TA, Ovchinnikov YA (1973) Sandwich complexes as a functional fonn of the enniatin ionophores. Febs Letters 1973; 36: 65-71.CrossRefGoogle Scholar
  12. 12.
    Benz R. Alkali ion transport through lipid bilayer membranes mediated by enniatin A and B and beauvericin. J Membr Biol 1978; 43: 367-394.CrossRefPubMedGoogle Scholar
  13. 13.
    Ojcius DM, Zychlinsky A, Zheng LM, Young JD Ionophore-induced apoptosis: role of DNA fragmentation and calcium fluxes. Exp Cell Res 1991; 197: 43-49.PubMedGoogle Scholar
  14. 14.
    Prince RC, Crofts AR, Steinrauf LK. A comparison of beauvericin, enniatin and valinomycin as calcium transporting agents in liposomes and chromatophores. Biochem Biophys Res Comm 1974; 59: 697-703.CrossRefPubMedGoogle Scholar
  15. 15.
    Dorschner E, Lardy H. Antimicrobial Agents and Chemotherapy 1968: 11-14.Google Scholar
  16. 16.
    Roeske RW, Isaac S, King TE, Steinrauf LK. The binding of barium and calcium ions by the antibiotic beauvericin. Biochem Biophys Res Comm 1974; 57: 554-561.CrossRefPubMedGoogle Scholar
  17. 17.
    Nakajyo S, Matsuoka K, Kitayama T, Yamamura Y, Shimizu K, Kimura M, Urakawa N. Inhibitory effect of beauvericin on a high KC-induced tonic contraction in guinea-pig taenia coli. Jpn J Pharmacol 1987; 45: 317-325.PubMedGoogle Scholar
  18. 18.
    Krska R, Schuhmacher R, Grasserbauer M, Lemmens M, Lemmens-Gruber R, Adler A, Lew H. Effects of beauvericin to mammalian tissue and its production by Austrian isolates of Fusarium proliferatum and Fusarium subglutinans. Mycotoxin Res 1997; 13: 11-16.Google Scholar
  19. 19.
    Reiter M. Die Wertbestimmung inotrop wirkender Arzneimittel am isolierten Papillarmuskel. Arzneim Forsch 1967; 17: 1249-1253.Google Scholar
  20. 20.
    Näbauer M, Callewaert G, Cleemann L, Morad M. Regulation of calcium release is gated by calcium current, not gating charge, in cardiac myocytes. Science 1989; 244: 800-803.PubMedGoogle Scholar
  21. 21.
    Nakajima H, Hoshiyama M, Yamashita K, Kiyomoto A. Effect of diltiazem on electrical and mechanical activity of isolated cardiac ventricular muscle of guinea pig. Jpn J Pharmacol 1975; 25: 383-392.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • R. Lemmens-Gruber
    • 1
  • B. Rachoy
    • 1
  • E. Steininger
    • 1
  • K. Kouri
    • 1
  • P. Saleh
    • 1
  • R. Krska
    • 2
  • R. Josephs
    • 2
  • M. Lemmens
    • 3
  1. 1.Institute of Pharmacology and ToxicologyUniversity of ViennaViennaAustria
  2. 2.Center for Analytical ChemistryInstitute for Agrobiotechnology (IFA-Tulln)TullnAustria
  3. 3.Department of Plant Production BiotechnologyTullnAustria

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