Molecular and Cellular Biochemistry

, Volume 204, Issue 1–2, pp 49–56

Selenoprotein W accumulates primarily in primate skeletal muscle, heart, brain and tongue

  • Q-P Gu
  • Y. Sun
  • L.W. Ream
  • P.D. Whanger
Article

DOI: 10.1023/A:1007065829068

Cite this article as:
Gu, QP., Sun, Y., Ream, L. et al. Mol Cell Biochem (2000) 204: 49. doi:10.1023/A:1007065829068

Abstract

The human selenoprotein W coding region with the selenocysteine codon (TGA) changed to a cysteine codon (TGT) was fused to six histidine codons (at its 3′ end), cloned into a prokaryotic expression vector (pTrc99a), and the corresponding mutated selenoprotein W was expressed in bacteria. The protein was purified by Ni-NTA agarose column and reverse phase HPLC. Polyclonal antibodies raised against this protein were used in Western blots to determine tissue distribution of selenoprotein W from rhesus monkeys fed a commercial chow. Selenoprotein W was found in several tissues with highest amounts in skeletal muscle and heart (muscle 6 fold greater than liver) and lowest levels in liver, but selenium concentrations were highest in kidneys (10 fold greater than muscle) and lowest in skeletal muscle. Northern blots using a human selenoprotein W cDNA probe indicated that mRNA levels were highest in monkey skeletal muscle and heart (2-2.5 fold greater than in liver), which is similar to the pattern found with a human multiple tissue Northern blot. However, as in the monkey, selenium concentrations were highest in human kidney and lowest in skeletal muscle and heart. Thus, selenoprotein W protein levels correlated with selenoprotein W mRNA levels but not with tissue selenium concentrations.

selenoprotein W primate tissues Northern and Western blots mutated selenoprotein W bacteria 

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Q-P Gu
    • 1
  • Y. Sun
    • 1
  • L.W. Ream
    • 2
  • P.D. Whanger
    • 1
  1. 1.Departments of Environmental and Molecular ToxicologyOregon State UniversityCorvallisUSA
  2. 2.Department of MicrobiologyOregon State UniversityCorvallisUSA

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