Molecular Biology Reports

, Volume 26, Issue 3, pp 185–194 | Cite as

The homeodomain transcription factor CDP/cut interacts with the cell cycle regulatory element of histone H4 genes packaged into nucleosomes

  • Thomas J. Last
  • André J. van Wijnen
  • Marleen C. de Ridder
  • Gary S. Stein
  • Janet L. Stein
Article

Abstract

The homeodomain transcription factor CDP/cut contains four separate DNA binding domains and interacts with large segments of DNA. Thus, CDP/cut has the potential to function as an architectural protein and perhaps to support modifications in chromatin structure and nucleosomal organization. To begin to examine the ability of CDP/cut to interact with chromatin, we analyzed binding of CDP/cut to the histone H4 gene promoter (−90 to +75) reconstituted into nucleosome cores. The −90 to +75 region encompasses the cell cycle regulatory element (Site II) that controls histone H4 gene transcription, a CDP/cut binding site and a nuclease hypersensitive region. Using electrophoretic mobility shift assays and DNase I footprinting experiments, we show that CDP/cut specifically interacts with its recognition motif in a nucleosomal context without displacing the nucleosome core. The competency of CDP/cut to interact with nucleosomes suggests that this transcription factor may facilitate chromatin remodeling in response to cell cycle regulatory and/or developmental cues.

cell cycle chromatin histone homeodomain nucleosome tanscription 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Thomas J. Last
    • 1
  • André J. van Wijnen
    • 1
  • Marleen C. de Ridder
    • 1
  • Gary S. Stein
    • 1
  • Janet L. Stein
    • 1
  1. 1.Department of Cell BiologyUniversity of Massachusetts Medical SchoolWorcesterUSA (Phone

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