Mycopathologia

, 146:99

The occurrence of ochratoxin A in dust collected from a problem household

  • John L. Richard
  • Ronald D. Plattner
  • Jeff May
  • Sandra L. Liska
Article

Abstract

Accumulated dust samples were collected from the heating ducts in a household where signs resembling ochratoxin poisoning in animals occurred. Several Penicillium spp. and Aspergillus ochraceous had been identified previously from air samples taken from this house. A composite sample from six collected samples was examined by HPLC, and it was determined that 58 ppb of ochratoxin A was present in this sample. A second set of six samples was collected and determinations were made by HPLC of the ochratoxin content in each sample. All samples, including one sample of dirt from a crawl space, yielded at least a trace of ochratoxin A; however, one sample of dust collected from the heating ducts yielded over 1500 ppb of ochratoxin A, and another sample of dust from a different heating duct yielded 306 ppb of ochratoxin A. Ochratoxin A was confirmed in all samples by LC-MS, and ochratoxin was evident in the samples by TLC analysis. This is believed to be the first report of finding ochratoxin inhouse dust.

environmental hazards house dust indoor air quality mycotoxin analysis by HPLC and LC-MS ochratoxicosis 

References

  1. 1.
    Van der Merwe KJ, Steyn PS, Fourie L, Scott DB, Theron JJ. Ochratoxin A, a metabolite produced by Aspergillus ochraceus Wilh. Nature 1965: 205: 1112–1113.PubMedCrossRefGoogle Scholar
  2. 2.
    Harwig J, Kuiper-Goodman T, Scott PM. Microbial food toxicants: Ochratoxins. In: Rechcigl M, ed. Handbook of foodborne diseases of biological origin, Boca Raton, FL: CRC Press, 1983:193–238.Google Scholar
  3. 3.
    Kuiper-Goodman T, Scott PM. Risk assessment of the mycotoxin ochratoxin A. Biomed Environ Sci 1989; 2: 179–248.PubMedGoogle Scholar
  4. 4.
    Abarca ML, Bragulat MR, Castella G, Cabanes FJ. Ochratoxin A production by strain of Aspergillus niger var. niger. Appl Environ Microbiol 1994; 60: 2650–2652.PubMedGoogle Scholar
  5. 5.
    Shotwell OL, Hessekine CW, Goulden ML. Note on the natural occurrence of ochratoxin A. J Assoc Off Anal Chem 1969; 52: 81–83.Google Scholar
  6. 6.
    Kuiper-Goodman T, Scott PM. Risk assessment of the mycotoxin ochratoxin A. Biomed Environ Sci 1989; 2: 179–248.PubMedGoogle Scholar
  7. 7.
    MacDonald S, Wilson P, Barnes K, Damant A, Massey R, Mortby E, Shepherd MJ. Ochratoxin A in dried fruits, method development and survey. Food Additi Contam 1999; 6: 253–260.CrossRefGoogle Scholar
  8. 8.
    Zimmerli D, Dick R. Ochratoxin A in table wine and grape juice: Occurrence and risk assessment. Food Addit Contam 1996; 13: 655–668.PubMedGoogle Scholar
  9. 9.
    Krogh P, Hald B, Pedersen J. Occurrence of ochratoxin A and citrinin in cereals associated with mycotoxic porcine nephropathy. Acta Path Microbiol Scand 1973; 81 Sect. B: 689–695.Google Scholar
  10. 10.
    Krogh P, Axelsen NH, Elling F, Gyrd-Hasssen N, Hald B, Hyldgarrd-Jensen J, Larsen AE, Madsen A, Mortensen HP, Moller T, Petersen OK, Ravnskov U, Rostgasrd M, Aalund O. Experimental porcine nephropathy – Changes of renal func103 tion and structure induced by ochratoxin A-contaminated feed. Acta Path Microbiol Scand 1974; 246 Sect. A (suppl 1): 14.Google Scholar
  11. 11.
    NTP. NTP Technical Report on the toxicology and carcinogenesis studies of ochratoxin A (CAS No.303-47-9) in F344/N rats (Gavage Studies). In: NIH publication No. 89-2813. Research Triangle Park, NC: U.S. Dept. Health and Human Services, National Institutes of Health, 1989.Google Scholar
  12. 12.
    Boorman GA, McDonald MR, Imoto S, Persing R. Renal lesions induced by ochratoxin A exposure in the F344 rat. Toxicol Pathol 1992; 20: 236–245.PubMedCrossRefGoogle Scholar
  13. 13.
    Castegnaro M, Plestina R, Dirheimer O, Chernosemsky IN, Barcsch H (eds). Mycotoxins, Endemic Nephropathy and Urinary Tract Tumors. IARC Sci Publ 1991; 115: 1–340.Google Scholar
  14. 14.
    Golinski P, Grabarkiewicz-Szczesna J, Chelkowski J, Hult K. Kostecki M. Possible sources of ochratoxin A in human blood in Poland. In: Castegnaro M, Plestina R. Dirheimer G, Chermozemsky IN, Barsch H, eds. Mycotoxins, endemic nephropathy and urinary tract tumors, Lyon: IARC, 1991: 153–158.Google Scholar
  15. 15.
    Hald B. Ochratoxin A in human blood in European countries. In: Castegnaro M, Plestina R. Dirheimer G, Chermozemsky IN, Barsch H, eds. Mycotoxins, endemic nephropathy and urinary tract tumors, Lyon: IARC, 1991: 159–164.Google Scholar
  16. 16.
    Creppy EE, Betbeder AM, Gharbi A, Counord J, Castegnaro M, Bartsch H, Moncharrnont P, Fouillet B, Chambon P, Dirheimer G. Human ochratoxicosis in France. In: Castegnaro M, Plestina R. Dirheimer G, Chermozemsky IN, Barsch H, eds. Mycotoxins, endemic nephropathy and urinary tract tumors, Lyon: IARC, 1991: 145–151.Google Scholar
  17. 17.
    Frolich AA, Marquardt RR, Ominsk, KH. Ochratoxin A as a contaminant in the human food chain: A Canadian perspective. In: Castegnaro M, Plestina R. Dirheimer G, Chermozemsky IN, Barsch H, eds. Mycotoxins, endemic nephropathy and urinary tract tumors, Lyon: IARC, 1991: 139–143.Google Scholar
  18. 18.
    Petkova-Bocharova T, Castegnaro M. Ochratoxin A in human blood in relation to Balkan Endemic Nephropathy and urinary tract tumours in Bulgaria. In: Castegnaro M, Plestina R. Dirheimer G, Chermozemsky IN, Barsch H, eds. Mycotoxins, endemic nephropathy and urinary tract tumors, Lyon: IARC, 1991: 135–137.Google Scholar
  19. 19.
    Fuchs R, Radic B, Ceovic S, Sostaric B, Hult K. Human exposure to ochratoxin A. In: Castegnaro M, Plestina R. Dirheimer G, Chermozemsky IN, Barsch H, eds. Mycotoxins, endemic nephropathy and urinary tract tumors, Lyon: IARC, 1991: 131–134.Google Scholar
  20. 20.
    Krogh P, Elling F, Hald B, Jylling B, Petersen VE, Skadhauge E, Svendsen CK. Changes in renal function and structure induced by ochratoxin A-contaminated feed. Acta Pathol Microbiol Scand 1976; 84: 215–221.Google Scholar
  21. 21.
    Lusky K, Gobel R. Tesch D, Tenner B, Halder W, Kruger M, Lippert A. Studies on the effects of ochratoxin A and deoxynivalenol toxicity on the health of pigs and tissue residue concentrations. Tierarztliche Umschau 1998; 53: 623–630.Google Scholar
  22. 22.
    DiPaolo N, Guarnieri A, Loi F, Sacchi G, Mangiarotti AM, DiPaolo M. Acute renal failure from inhalation of mycotoxins. Nephron 1993; 64: 621–625.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • John L. Richard
    • 1
  • Ronald D. Plattner
    • 2
  • Jeff May
    • 3
  • Sandra L. Liska
    • 4
  1. 1.RomerTM Labs, Inc.UnionUSA
  2. 2.National Center for Agricultural Utilization ResearchPeoriaUSA
  3. 3.May Home InspectionsCambridgeUSA
  4. 4.Harwich PortUSA

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