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Glycoconjugate Journal

, Volume 16, Issue 10, pp 629–638 | Cite as

Monosaccharide compositions of Danaus plexippus (monarch butterfly) and Trichoplusia ni (cabbage looper) egg glycoproteins

  • Yong-Il Park
  • H. Alan Wood
  • Yuan Chuan Lee
Article

Abstract

Monosaccharide compositions of eggs from Danaus plexippus (monarch butterfly) and Trichoplusia ni (cabbage looper) were analyzed. Analyses were performed mainly with high performance anion exchange chromatography (HPAEC) using crude extracts of eggs or SDS-PAGE separated and PVDF-blotted protein bands. Man and GlcN were the major components in all cases, but low levels of Gal and Fuc were possibly present in some samples. Some T. ni egg glycoproteins even contained GalN. Although a peak comigrating with Neu5Ac could be detected with HPAEC-PAD or RP-HPLC (fluorometry) after derivatization with 1,2-diamino-4,5-methylenedioxy-benzene, the quantities were too small to be significant as an integral part of the analyzed glycoproteins. These data suggests that most of glycans on the glycoproteins are pauci-Man type N-glycans, but a small portion of N-glycan may be either hybrid type or complex type.

insect eggs monarch butterfly cabbage looper glycoproteins 

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References

  1. 1.
    Davidson DJ, Castellino FJ (1991a) Asparagine-linked oligosaccharide processing in lepidopteran insect cells. Temporal dependence of the nature of the oligosaccharides assembled on asparagine-289 of recombinant human plasminogen produced in baculovirus vector infected Spodoptera frugiperda (IPLB-SF-21 AE) cells. Biochemistry 30: 6167–74.Google Scholar
  2. 2.
    Davidson DJ, Castellino FJ (1991b) Structures of the asparagine-289-linked oligosaccharides assembled on recombinant human plasminogen expressed in a Mamestra brassicae cell line (IZDMB0503). Biochemistry 30: 6689–96.Google Scholar
  3. 3.
    Davidson DJ, Fraser MJ, Castellino FJ (1990) Oligosaccharide processing in the expression of human plasminogen cDNA by lepidopteran insect (Spodoptera fugiperda) cells. Biochemistry 29: 5584–90.Google Scholar
  4. 4.
    Davis TR, Wood HA (1995) Intrinsic glycosylation potentials of insect cell cultures and insect larvae. In Vitro Cell. Dev. Biol. 31: 659–63.Google Scholar
  5. 5.
    Fan J-Q, Kondo A, Kato I, Lee YC (1994a) High performance liquid chromatography of glycopeptides and oligosaccharides on graphitized carbon columns. Anal. Biochem. 219: 224–9.Google Scholar
  6. 6.
    Fan J-Q, Namiki Y, Matsuoka K, Lee YC (1994b) Comparison of acid hydrolytic conditions for Asn-linked oligosaccharides. Anal. Biochem. 219: 375–8.Google Scholar
  7. 7.
    Hara S, Yamaguchi M, Takemori Y, Furuhata K, Ogura H, M. N (1989) Determination of mono-O-acetylated N-acetylneuraminic acids in human and rat sera by fluorometric high-performance liquid chromatography. Anal. Biochem. 179: 162–6.Google Scholar
  8. 8.
    Hard K, Van Doorn JM, Thomas-Oates JE, Kamerling JP, Van der Horst DJ (1993) Structure of the Asn-linked oligosaccharides of apolipophorin III from the insect Locusta migratoria. Carbohydrate-linked 2-aminoethylphosphonate as a constituent of a glycoprotein. Biochemistry 32: 766–75.Google Scholar
  9. 9.
    Honda S, Akao E, Suzuki S, Okuda M, Kakehi K, Nakamura J (1989) High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolone derivatives. Anal. Biochem. 180: 351–7.Google Scholar
  10. 10.
    Hsu TA, Watson S, Eiden JJ, Betenbaugh M (1996) Rescue of immunoglobulins from insolubility is facilitated by PDI in the baculovirus expression system. Prot. Expression and Purification 7: 281–8.Google Scholar
  11. 11.
    Hughes PR, Radke CD, Renwick JA (1993) A simple, low-input method for continuous laboratory rearing of the monarch butterfly (Lepidoptera: danaidea) for research. Amer. Entomologist 39: 109–11.Google Scholar
  12. 12.
    Hughes PR, Van-Beek NAM, Wood HA (1986) A modified droplet feeding method for rapid assay of Bacillus thuringiensis and baculoviruses in noctoid larvae. J. Invertebr. Pathol. 48: 187–92.Google Scholar
  13. 13.
    Kubelka V, Altman F, Kornfeld G, März L (1994) Structure of the N linked oligosaccharides of the membrane glycoproteins from three Lepidopteran cell Enes (Sf-21, IZD-Mb-0503, Bm-N). Arch. Biochem. Biophys. 308: 148–57.Google Scholar
  14. 14.
    Kubelka V, Altmann F, Staudacher E, Tretter V, März L, Hard K, Kamerling JP, Vliegenthart JFG (1993) Primary structures of the N-linked carbohydrate chains from honeybee venom phospholipase A2. Eur. J. Biochem. 213: 1193–204.Google Scholar
  15. 15.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–5.Google Scholar
  16. 16.
    Lucnow (1994) Protein production and processing from baculovirus expression vectors. In Baculovirus expression systems and biopesticides (Schuler ML, Wood HA, Granados RR, Hammer DA eds), pp 51–90. Wiley-Liss, Inc.: New York, NY.Google Scholar
  17. 17.
    März L, Altman F, Staudacher E, Kubelka V (1995) Protein glycosylation in insects. In Glycoproteins (Montreuil J, Schachter H, Vliegenthart H eds) vol 3, pp 543–63. Elsevier, B.V.: Amsterdam, The Netherlands.Google Scholar
  18. 18.
    McKelvy JF, Lee YC (1969) Microheterogeneity of the carbohydrate group of Aspergillus oryzae α-amylase. Arch. Biochem. Biophys. 132: 99–110.Google Scholar
  19. 19.
    Ogonah OW, Freedman RB, Jenkins N, Patel K, Rooney BC (1996) Isolation and characterization of an insect cell line able to perform complex N-linked glycosylation on recombinant proteins. Biotechnol. 14: 197–202.Google Scholar
  20. 20.
    Staudacher E, Kubelka V, März L (1992) Distinct N-glycan fucosylation potentials of three Lepidopteran cell lines. Eur. J. Biochem. 207: 987–93.Google Scholar
  21. 21.
    Weiss JB, Magnani JL, Strand M (1986) Identification of Schistosoma mansoni glycolipids that share immunogenic carbohydrate epitopes with glycoproteins. J. Immunol. 136: 4275–82.Google Scholar
  22. 22.
    Weitzhandler M, Hardy M, Co MS, Avdalovic N (1994) Analysis of carbohydrates on IgG preparations. J. Pharm. Sci. 83: 1670–5.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Yong-Il Park
    • 1
  • H. Alan Wood
    • 2
  • Yuan Chuan Lee
    • 1
  1. 1.Biology DepartmentJohns Hopkins UniversityBaltimoreMaryland
  2. 2.Boyce Thompson Institute for Plant ResearchCornell UniversityNew York

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