Arginine vasopressin increases the rate of protein synthesis in isolated perfused adult rat heart via the V1 receptor
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Arginine vasopressin (AVP) is known to contribute significantly to the pathogenesis of congestive heart failure and hypertension. However, little is known about its effect on the myocardium. The present study was conducted to determine whether AVP directly increases the rate of protein synthesis in isolated, perfused rat heart, and, if so, the mechanism involved. Elevation of the aortic pressure from 60 to 120 mmHg in perfused rat heart accelerated the rate of protein synthesis which was associated with increases in cAMP levels and Ca2+ uptake. AVP (100 μM) increased Ca2+ uptake and accelerated the rate of protein synthesis without a change in cAMP concentration. The latter events were inhibited by OPC-21268 (100 μM), a selective V1 receptor antagonist, or amiloride (100 μM), an inhibitor of the Na+/H+ exchange system. However, increases in cAMP concentrations, Ca2+ uptake, and rates of protein synthesis associated with the elevated aortic pressure were not inhibited by amiloride. Thus, AVP directly increased the rate of protein synthesis via the V1 receptor that is sensitive to amiloride, a mechanism that differs from the cAMP-dependent mechanism that is responsible for the cardiac hypertrophy induced by pressure overload.
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- 3.Watson PA, Haneda T, Morgan HE: Effect of higher aortic pressure on ribosome formation and CAMP content in rat heart. Am J Physiol C1257–C1261, 1989Google Scholar
- 7.Baker KM, Aceto JF: Angiotensin II stimulation of protein synthesis and cell growth in chick heart cells. Am J Physiol H610–H618, 1990Google Scholar
- 15.Haneda T, Watson PA, Morgan HE: Elevated aortic pressure, calcium uptake, and protein synthesis in rat heart. J Mol Cell Cardiol 1: 131–138, 1989Google Scholar
- 16.Sugden PH, Fuller SJ: Correlations between cardiac protein synthesis rates, intracellular pH and the concentrations of creatine metabolites. Biochem J 339–346, 1991Google Scholar
- 19.Cowley AJ, Cushman WC, Quillen EJ, Skeiton MM, Langford HG: Vasopressin elevation in essential hypertension and increased responsiveness to sodium intake. Hypertension I93–I100, 1981Google Scholar
- 26.Kira Y, Kochel PJ, Gordon EE, Morgan HE: Aortic perfusion pressure as a determinant of cardiac protein synthesis. Am J Physiol C247–C258, 1984Google Scholar
- 29.Kleyman TR, Cragoe EJ: Amiloride and its analogs as tools in the study of ion transport. J Mem Biol 105: 1–21, 1988Google Scholar
- 31.Okada K, Sasaki R, lshikawa S, Saito T: Distinct inhibition by nonpeptide and peptide arginine vasopressin antagonists of vasopressininduced activation of mitogen-activated protein kinase in cultured rat vascular smooth muscle cells. Biochem Biophys Res Commun 200: 1155–1160, 1994PubMedGoogle Scholar
- 32.Cheng CP, Igarashi Y, Klopfenstein HS, Applegate RJ, Shihabi Z, Little WC: Effect of vasopressin on left ventricular performance. Am J Physiol H53–H60, 1993Google Scholar
- 37.Moalic JM, Bauters C, Himbert D, Bercovici J, Mouas C, Guicheney P, Baudoin LM, Rappaport L, Emanoil RR, Mezger V, Swyngheadauw B: Phenylephrine, vasopressin and angiotensin II as determinants of protooncogene and heat-shock protein gene expression in adult rat heart and aorta. J Hypertens 7: 195–201, 1989PubMedGoogle Scholar