Molecular and Cellular Biochemistry

, Volume 194, Issue 1–2, pp 93–98

Anabolic effect of daidzein on cortical bone in tissue culture: Comparison with genistein effect

  • Ying Hua Gao
  • Masayoshi Yamaguchi
Article

Abstract

The effect of daidzein on cortical bone in vitro was investigated. Femoral-diaphyseal tissues obtained from elderly female rats were cultured for 24 h in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplementation with antibiotics and bovine serum albumin. The experimental cultures contained 10-7 to 10-5 M daidzein. The presence of daidzein (10-6 and 10-5 M) caused a significant increase of alkaline phosphatase activity, deoxyribonucleic acid (DNA) and calcium contents in bone tissues. This effect was equal to that of genistein (10-6 and 10-5 M). Daidzein (10-5 M) or genistein (10-5 M)-induced increase of calcium content and alkaline phosphatase activity in bone tissues was completely prevented by cycloheximide (10-6 M), an inhibitor of protein synthesis. Anabolic effect of daidzein and genistein on bone components was equal to that of 17β-estradiol (10-8 M). The effect of isoflavohoids was not enhanced by the addition of 17β-estradiol. The combination of daidzein and genistein did not have an additive effect. These findings indicate that daidzein has an anabolic effect on bone metabolism in tissue culture in vitro, and that this effect is equal to genistein effect. Isoflavonoids may stimulate bone formation and mineralization.

diadzein genistein estrogen bone metabolism cortical bone tissue culture 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Nishimoto SK, Chang C-H, Gendler E, Stryker WF, Nimni ME: The effect of aging on bone formation in rats: biochemical and histological evidence for decreased bone formation capacity. Calcif Tissue Int 37: 617–624, 1985PubMedGoogle Scholar
  2. 2.
    Schapira D, Linn S, Sarid M, Mokadi S, Kabala A, Silbermann M: Calcium and vitamin D enriched diets increase and preserve vertebral mineral content in aging laboratory rats. Bone 16: 575–582, 1995PubMedGoogle Scholar
  3. 3.
    Wild RA, Burchaman JR, Myers C, Demers LM: Declining adrenal androgen: an association with bone loss in aging women. Proc Soc Exp Biol Med 186: 355–360, 1987PubMedGoogle Scholar
  4. 4.
    Young GA, Chem C, Hill GL: Assessment of protein-caloric malnutrition in surgical patients from plasma proteins and anthropometric measurements. Am J Clin Nutr 31: 429–435, 1980Google Scholar
  5. 5.
    Bonjour J-P, Schurch M-A, Rizzoli R: Nutritional aspects of hip fractures. Bone 18: 139s–144s, 1996PubMedGoogle Scholar
  6. 6.
    Keung WM, Klyosov AA, Vallee BL: Daidzin inhibits mitochondrial aldehyde dehydrogenase and suppress ethanol intake of Syrian golden hamsters. Proc Nat Acad Sci USA 94: 1675–1679, 1997PubMedGoogle Scholar
  7. 7.
    Liu Y, Bhalla K, Hill C, Priest DG: Evidence for involvement of tyrosine phosphorylation in taxol induced apoptosis in a human ovarian tumor cell line. Biochem Pharmacol 48: 1265–1272, 1994PubMedGoogle Scholar
  8. 8.
    Spinozzi F, Pagliacci MC, Mikliorati G, Moraca R, Grignami F, Riccardi C, Nicoletti I: The natural tyrosine kinase inhibitor genistein produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells. Leuk Res 18: 431–439, 1994PubMedGoogle Scholar
  9. 9.
    Blair HC, Jordan SE, Peterson TG, Barnes S: Variable effects of tyrosine kinase inhibitors on avian osteoclastic activity and reduction of bone loss in ovariectomized rats. J Cell Biochem 61: 629–637, 1996PubMedGoogle Scholar
  10. 10.
    Arjmandi BH, Alekei L, Hollis B, Amin D, Stacewicz-Sapuntzakis M, Guo P, Kukreja SC: Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis. J Nutr 126: 161–167, 1996PubMedGoogle Scholar
  11. 11.
    Yamaguchi M, Gao YH: Anabolic effect of genistein on bone metabolism in the femoral-metaphyseal tissues of elderly rats is inhibited by the antiestrogen tamoxifen. Res Exp Med 197: 101–107, 1997Google Scholar
  12. 12.
    Yamaguchi M, Gao YH: Inhibitory effect of genistein on bone resorption in tissue culture. Biochem Pharmacol 55: 71–76, 1998PubMedGoogle Scholar
  13. 13.
    Yamaguchi M, Oishi H, Suketa Y: Stimulatory effect of zinc on bone formation in tissue culture. Biochem Pharmacol 36: 4007–4012, 1987PubMedGoogle Scholar
  14. 14.
    Walter K, Schutt C: Acid and alkaline phosphatase in serum. In: HU Bergmeyer (ed). Methods of Enzymatic Analysis, vols 1 and 2, Academic Press, New York, 1965, pp 856–860Google Scholar
  15. 15.
    Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–273, 1951PubMedGoogle Scholar
  16. 16.
    Flanagan B, Nichols G Jr: Metabolic studies of bone in vitro. VI. Collagen biosynthesis by surviving bone fragment in vitro. J Biol Chem 237: 3786–3789, 1962PubMedGoogle Scholar
  17. 17.
    Ceriotti G: Determination of nucleic acids in animal tissues. J Biol Chem 214: 39–77, 1955Google Scholar
  18. 18.
    Hashizume M, Yamaguchi M: Effect of β-alanyl-L-histidinato zinc on differentiation of osteoblastic MC3T3-E1 cells: Increases in alkaline phosphatase activity and protein concentration. Mol Cell Biochem 131: 19–24, 1994PubMedGoogle Scholar
  19. 19.
    Yamaguchi M, Matsui T: Zinc enhancement of 17β-estradiol's anabolic effect in osteoblastic MC3T3-E1 cells. Calcif Tissue Int 60: 527–532, 1997PubMedGoogle Scholar
  20. 20.
    Yamaguchi M, Kishi S, Hashizume M: Effect of zinc-chelating dipeptides on osteoblastic MC3T3-E1 cells: Activation of aminoacyl-tRNA synthetase. Peptides 15: 1367–1371, 1994PubMedGoogle Scholar
  21. 21.
    Pain VN: Initiation of protein synthesis in mammalian cells. Biochem J 235: 625–637, 1986PubMedGoogle Scholar
  22. 22.
    Gao YH, Yamaguchi M: Zinc enhancement of genistein's anabolic effect on bone components in female elderly rats. Gen Pharmacol 31: 199–202, 1998PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Ying Hua Gao
    • 1
  • Masayoshi Yamaguchi
    • 1
  1. 1.Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional SciencesUniversity of ShizuokaShizuoka CityJapan

Personalised recommendations