Glycoconjugate Journal

, Volume 16, Issue 1, pp 19–26 | Cite as

Structural study of fucoidan from Cladosiphon okamuranus tokida

  • Masato Nagaoka
  • Hideyuki Shibata
  • Itsuko Kimura-Takagi
  • Shusuke Hashimoto
  • Kazumasa Kimura
  • Takashi Makino
  • Ritsuo Aiyama
  • Sadao Ueyama
  • Teruo Yokokura
Article

Abstract

A structural study was carried out on a fucoidan isolated from the brown seaweed Cladosiphon okamuranus. The polysaccharide contained fucose, glucuronic acid and sulfate in a molar ratio of about 6.1 : 1.0 : 2.9. The results of Smith degradation showed that this polysaccharide has a linear backbone of 1→3-linked α-fucopyranose with a half sulfate substitution at the 4-positions, and a portion of the fucose residues was O-acetylated. The data obtained from partial acid hydrolysis, a methylation analysis and NMR spectra indicated that the α-glucuronic acid residue is linked to the 2-positions of the fucose residues, which were not substituted by a sulfate group. These results indicated that the average structure of this fucoidan is as follows: -[(→3Fuc-4(±OSO3-)α1−)5→3[GlcAα1→2]Fucα1−]n−. (Half of each fucose residue was sulfated. One O-acetyl ester was present in every 6 fucose residues.)

fucoidan sulfated fucan seaweed 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Masato Nagaoka
    • 1
  • Hideyuki Shibata
    • 1
  • Itsuko Kimura-Takagi
    • 1
  • Shusuke Hashimoto
    • 1
  • Kazumasa Kimura
    • 1
  • Takashi Makino
    • 1
  • Ritsuo Aiyama
    • 1
  • Sadao Ueyama
    • 1
  • Teruo Yokokura
    • 1
  1. 1.Yakult Central Institute for Microbiological ResearchTokyoJapan

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