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Molecular and Cellular Biochemistry

, Volume 199, Issue 1–2, pp 139–147 | Cite as

Specific down-regulation of annexin II expression in human cells interferes with cell proliferation

  • Yangping Chiang
  • Angie Rizzino
  • Zita A. Sibenaller
  • Marc S. Wold
  • Jamboor K. Vishwanatha
Article

Abstract

The protein-tyrosine kinase substrate annexin II is a growth regulated gene whose expression is increased in several human cancers. While the precise function of this protein is not understood, annexin II is proposed to be involved in multiple physiological activities, including DNA synthesis and cell proliferation. Targeted disruption of the annexin II gene affects calcium signaling, tyrosine phosphorylation and apoptosis, indicating the important physiological role of this protein. We used a transient co-transfection assay to regulate annexin II expression in human HeLa, 293 and 293T cells, and measured the effects of annexin II down regulation on DNA synthesis and proliferation. Transfection of cells with an antisense annexin II vector results in inhibition of cell division and proliferation, with concomitant reduction in annexin II message and protein levels. Cellular DNA synthesis is significantly reduced in antisense transfected cells. Replication extracts made from antisense transfected cells have significantly reduced efficiency to support SV40 in vitro DNA replication, while the extracts made from sense transfected cells are fully capable of replication. Our results indicate an important role of annexin II in cellular DNA synthesis and cell proliferation.

annexin II DNA synthesis antisense Simian Virus 40 cell proliferation 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Yangping Chiang
    • 1
  • Angie Rizzino
    • 2
  • Zita A. Sibenaller
    • 3
  • Marc S. Wold
    • 3
  • Jamboor K. Vishwanatha
    • 1
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of Nebraska Medical CenterOmahaUSA
  2. 2.Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical CenterOmahaUSA
  3. 3.Department of BiochemistryUniversity of Iowa College of MedicieIowa CityUSA

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