Mycopathologia

, Volume 142, Issue 3, pp 161–167 | Cite as

in Vitro Effect of Deoxynivalenol on the Differentiation of Human Colonic Cell Lines Caco-2 and t84

  • Fumiko Kasuga
  • Yukiko Hara-Kudo
  • Noriko Saito
  • Susumu Kumagai
  • Yoshiko Sugita-Konishi
Article

Abstract

The effects of low concentrations of deoxynivalenol (DON) on structural and functional characteristics of human colonic adenocarcinoma cell lines Caco-2 and T84 were examined. Scanning electron microscopic (SEM) analysis of the apical surfaces of Caco-2 cells revealed reduction or abnormal formation of brush borders in the presence of 50, 100 and 200 ng/ml of DON. Monolayer integrity of Caco-2 and T84 cells was studied using cells which were cultured on permeable membranes. The transepithelial electrical resistance (TEER) of Caco-2 cells was significantly reduced at 50, 100 and 200 ng/ml of DON, significant increase in lucifer yellow (LY) permeability was also observed in these cells at 100 ng/ml of DON. The TEER of T84 cells was significantly reduced at 100 and 200 ng/ml of DON. LY permeability significantly increased at 200 ng/ml of DON in T84 cells. Enzyme activities in Caco-2 cells were also examined. Alkaline phosphatase activity was reduced from the 6th to 15th day of culture in the presense of 100 or 200 ng/ml of DON, whereas sucrase- isomaltase activity was significantly decreased by adding 50 or 100 ng/ml of DON for 15 or 20 days. Protein content was attenuated only by treatment with 200 ng/ml of DON thoughout the experimental period. The results indicate that DON interferes with structural and functional characteristics of differentiation in enterocytes at low doses.

Caco-2 cells deoxynivalenol differentiation enterocytes T84 cells 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Fumiko Kasuga
    • 1
  • Yukiko Hara-Kudo
    • 1
  • Noriko Saito
    • 2
  • Susumu Kumagai
    • 1
  • Yoshiko Sugita-Konishi
    • 1
  1. 1.Department of Biomedical Food ResearchNational Institute of Infectious DiseasesTokyoJapan
  2. 2.Laboratory of ElectronmicroscopyNational Institute of Infectious DiseasesTokyoJapan

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