Glycoconjugate Journal

, Volume 15, Issue 2, pp 139–145 | Cite as

Combined preparative enzymatic synthesis of dTDP-6-deoxy-4-keto-D-glucose from dTDP and sucrose

  • Andreas Stein
  • Maria-Regina Kula
  • Lothar Elling

Abstract

dTDP–6–deoxy–4–keto–D–glucose (1), the common intermediate in the biosyntheses of the mainfold deoxysugars, was synthesized on a gram–scale by the combination of sucrose synthase and dTDP–D–glucose 4,6–dehydratase in a fed batch, starting the reaction with dTDP. This process allowed a dTDP conversion with a 100% rate. An easy and efficient three–step purification with anion–exchange chromatography and gel filtration gave 1.1 g of 1 in an overall yield of 73%. This work realizes a first step for an economic access to activated deoxysugars.

activated deoxysugars preparative enzymatic synthesis sucrose synthase dehydratase 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Andreas Stein
    • 1
  • Maria-Regina Kula
    • 1
  • Lothar Elling
    • 1
  1. 1.Institue of Enzyme TechnologyHeinrich-Heine-University Düsseldorf in the research centre JülichJülichGermany

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