Molecular and Cellular Biochemistry

, Volume 199, Issue 1–2, pp 103–109 | Cite as

Fluoxetine interacts with the lipid bilayer of the inner membrane in isolated rat brain mitochondria, inhibiting electron transport and F1F0-ATPase activity

  • Carlos Curti
  • Fábio E. Mingattao
  • Ana C.M. Polizello
  • Leandro O. Galastri
  • Sérgio A. Uyemura
  • Antonio C. Santos
Article

Abstract

The effects of fluoxetine on the oxidative phosphorylation of mitochondria isolated from rat brain and on the kinetic properties of submitochondrial particle F1F0-ATPase were evaluated. The state 3 respiration rate supported by pyruvate + malate, succinate, or ascorbate + tetramethyl-p-phenylenediamine (TMPD) was substantially decreased by fluoxetine. The IC50 for pyruvate + malate oxidation was ∼ 0.15 mM and the pattern of inhibition was the typical one of the electron-transport inhibitors, in that the drug inhibited both ADP- and carbonyl cyanide m-chlorophenylhydrazone (CCCP)-stimulated respirations and the former inhibition was not released by the uncoupler. Fluoxetine also decreased the activity of submitochondrial particle F1F0-ATPase (IC50 ∼ 0.08 mM) even though K0.5 and activity of Triton X-100 solubilized enzyme were not changed substantially. As a consequence of these effects, fluoxetine decreased the rate of ATP synthesis and depressed the phosphorylation potential of mitochondria. Incubation of mitochondria or submitochondrial particles with fluoxetine under the conditions of respiration or F1F0-ATPase assays, respectively, caused a dose-dependent enhancement of 1-anilino-8-naphthalene sulfonate (ANS) fluorescence. These results show that fluoxetine indirectly and nonspecifically affects electron transport and F1F0)-ATPase activity inhibiting oxidative phosphorylation in isolated rat brain mitochondria. They suggest, in addition, that these effects are mediated by the drug interference with the physical state of lipid bilayer of inner mitochondrial membrane.

antidepressive agents fluoxetine rat brain mitochondria oxidative phosphorylation H(+)transporting-ATP synthase ATP synthesis ATP hydrolysis 1-aniline-8-naphathalene sulfonate fluorescence 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Carlos Curti
    • 1
  • Fábio E. Mingattao
    • 1
  • Ana C.M. Polizello
    • 1
  • Leandro O. Galastri
    • 2
  • Sérgio A. Uyemura
    • 2
  • Antonio C. Santos
    • 2
  1. 1.Department of Physics and Chemistry, School of Pharmaceutical SciencesUniversity of São PauloSão PauloBrasil
  2. 2.Department of Clinical, Toxicological and Bromatological Analysis, School of Pharmaceutical SciencesUniversity of São PauloSão PauloBrasil

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