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Molecular and Cellular Biochemistry

, Volume 173, Issue 1–2, pp 1–5 | Cite as

Transferrin in cultured human term cytotrophoblast cells: Synthesis and heterogeneity

  • C.E.H. Verrijt
  • M.J. Kroos
  • A.J.M. Verhoeven
  • H.G. van Eijk
  • J.P. van Dijk
Article

Abstract

Transferrin (Tf) mRNA was recently demonstrated in rat and mouseplacental tissue. Rat placental cells were shown to secrete transferrin. Thecell type with which Tf mRNA was associated was not investigated. Wetherefore studied the ability of immunopurified human term cytotrophoblastcells in culture to synthesize Tf, by means of pulse-label experiments with35S-methionine and report that these cells do synthesize Tf. Tf mRNA wasdemonstrated in the cell lysates by means of RT-PCR. Tf isolated fromcytotrophoblast and syncytiotrophoblast cells was shown to be different fromboth maternal and fetal serum Tf with respect to the distribution ofisoforms as demonstrated by means of iso-electric focusing. The iso-electricpoints were found at lower pH values (pH 5.0-5.4), compared to theiso-electric points of maternal and fetal serum Tf, suggesting a higherdegree of sialylation and glycan chain complexity.

human cytotrophoblast syncytiotrophoblast culture iso-transferrins synthesis 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • C.E.H. Verrijt
    • 1
  • M.J. Kroos
    • 1
  • A.J.M. Verhoeven
    • 2
  • H.G. van Eijk
    • 1
  • J.P. van Dijk
    • 1
  1. 1.Department of Chemical PathologyErasmus University RotterdamRotterdamThe Netherlands
  2. 2.Department of BiochemistryErasmus University RotterdamRotterdamThe Netherlands

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