Molecular and Cellular Biochemistry

, Volume 173, Issue 1–2, pp 1–5 | Cite as

Transferrin in cultured human term cytotrophoblast cells: Synthesis and heterogeneity

  • C.E.H. Verrijt
  • M.J. Kroos
  • A.J.M. Verhoeven
  • H.G. van Eijk
  • J.P. van Dijk


Transferrin (Tf) mRNA was recently demonstrated in rat and mouseplacental tissue. Rat placental cells were shown to secrete transferrin. Thecell type with which Tf mRNA was associated was not investigated. Wetherefore studied the ability of immunopurified human term cytotrophoblastcells in culture to synthesize Tf, by means of pulse-label experiments with35S-methionine and report that these cells do synthesize Tf. Tf mRNA wasdemonstrated in the cell lysates by means of RT-PCR. Tf isolated fromcytotrophoblast and syncytiotrophoblast cells was shown to be different fromboth maternal and fetal serum Tf with respect to the distribution ofisoforms as demonstrated by means of iso-electric focusing. The iso-electricpoints were found at lower pH values (pH 5.0-5.4), compared to theiso-electric points of maternal and fetal serum Tf, suggesting a higherdegree of sialylation and glycan chain complexity.

human cytotrophoblast syncytiotrophoblast culture iso-transferrins synthesis 


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  1. 1.
    Aldred AR, Dickson PW, Marley PD, Schreiber G: Distribution of transferrin synthesis in brain and other tissues in the rat. J Biol Chem 262(11): 5293–5297, 1987Google Scholar
  2. 2.
    Yang F, Friedrichs WE, Buchanan JM, Herbert DC, Weaker FJ, Brock JH, Bowman BH: Tissue specific expression of mouse transferrin during development and aging. Mech Ageing Dev 56: 1187–1197, 1990Google Scholar
  3. 3.
    Boockfor FR, Harris SE, Barto JM, Bonner JM: Placental cell release of transferrin: Analysis by reverse hemolytic plaque assay. Placenta 15(5): 501–509, 1994Google Scholar
  4. 4.
    Kliman HJ, Nestler JE, Sernasi E, Sanger JM, Strauss JF III: Purification and in vitrodifferentiation of cytotrophoblasts from human term placentae. Endocrinology 118: 1567–1582, 1986Google Scholar
  5. 5.
    Starreveld JS, Kroos MJ, Verrijt CEH, van Eijk HG, van Dijk JP: Ferritin in cultured human cytotrophoblasts: Synthesis and subunit distribution. Placenta 16: 383–395, 1995Google Scholar
  6. 6.
    van Eijk HG, van Noort WL: The analysis of human transferrin with the PhastSystem: Quantitation of microheterogeneity. Electrophoresis 13: 354–358, 1992Google Scholar
  7. 7.
    Chomczynski P, Sacchi N: Single-step of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159, 1987Google Scholar
  8. 8.
    de Smit MH, Hoefkens P, de Jong G, van Duin J, van Knippenberg PH, van Eijk HG: Optimized bacterial expression of nonglycosylated human transferrin and its half-molecules. Int J Biochem Cell Biol 27: 839–850, 1995Google Scholar
  9. 9.
    Yang F, Lum JB, McGill JR, Moore CM, Naylor SL, van Bragt PH, Baldwin WD, Bowman BH: Human transferrin: cDNA characterization and chromosomal location. Proc Natl Acad Sci USA 81: 2752–2756, 1984Google Scholar
  10. 10.
    Galbraith GMP, Galbraith RM, Faulk WP: Immunological studies of transferrin and transferrin receptor of human placental trophoblast. Placenta 1: 33–46, 1980Google Scholar
  11. 11.
    Douglas GC, King BF: Uptake and processing of 125-I labelled transferrin and 59-Fe labelled transferrin by isolated human trophoblast cells. Placenta 11: 41–57, 1990Google Scholar
  12. 12.
    Starreveld JS, van Denderen J, Kroos MJ, van Dijk JP, van Eijk HG: The effect of iron supplementation on iron uptake by differentiating cytotrophoblasts. Reprod Fertil Dev 8: 417–422, 1996Google Scholar
  13. 13.
    Eaton BM, Browne MJ, Contractor SF: Transferrin mediated iron transport in the perfused isolated human placental lobule. Contrib Gynecol Obstet 13: 149–150, 1985Google Scholar
  14. 14.
    de Jong G, van Dijk JP, van Eijk HG: The Biology of Transferrin: A critical review. Clin Chim Acta 190: 146, 1990Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • C.E.H. Verrijt
    • 1
  • M.J. Kroos
    • 1
  • A.J.M. Verhoeven
    • 2
  • H.G. van Eijk
    • 1
  • J.P. van Dijk
    • 1
  1. 1.Department of Chemical PathologyErasmus University RotterdamRotterdamThe Netherlands
  2. 2.Department of BiochemistryErasmus University RotterdamRotterdamThe Netherlands

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