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Mycopathologia

, Volume 138, Issue 1, pp 29–35 | Cite as

Effect of iron on fluconazole activity against Candida albicans in presence of human serum or monocyte-derived macrophages

  • Yuriko Minn
  • Elmer Brummer
  • David A. Stevens
Article

Abstract

Human serum, transferrin, and apotransferrin are known to profoundly inhibit the growth of Candida albicans by iron deprivation. On the other hand, iron overload (iron saturated transferrin) is a serious risk factor for candidiasis in newborn and in leukemic patients. We tested the efficacy of fluconazole and the previously demonstrated synergy of fluconazole and effector cells against C. albicans under iron overload conditions where efficacy might be diminished. We confirm that exogenous iron completely reversed the inhibitory effect of human serum and report that the efficacy of fluconazole against C. albicans was not significantly compromised in a 24 h assay system. Although exogenous iron inhibited fungistatic activity of monocyte-derived macrophages, it did not interfere with the synergistic candidacidal activity of fluconazole and monocyte-derived macrophages. In 72 h assays, where fluconazole had candidacidal activity, exogenous iron did not compromise efficacy of fluconazole, and fluconazole activity was often increased. These in vitro results suggest that effectiveness of fluconazole therapy would not be compromised in iron overload situations in vivo.

Fluconazole Iron Candida albicans macrophage monocyte-derived 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Yuriko Minn
    • 1
    • 2
    • 3
  • Elmer Brummer
    • 1
    • 2
    • 3
  • David A. Stevens
    • 1
  1. 1.Division of Infectious Diseases, Department of MedicineSanta Clara Valley Medical CenterUSA
  2. 2.California Institute for Medical ResearchSan JoseUSA
  3. 3.Division of Infectious Diseases and Geographic MedicineStanford University Medical SchoolStanfordUSA

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