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Molecular and Cellular Biochemistry

, Volume 187, Issue 1–2, pp 133–139 | Cite as

Regulation of low density lipoprotein receptor mRNA levels by estradiol 17β and chorionic gonadotropin in human placenta

  • Y. Gopi Shanker
  • Usha P. Shetty
  • A. Jagannadha Rao
Article

Abstract

Inhibition of synthesis of estradiol 17β by the addition of inhibitors of aromatase, a key enzyme in the biosynthesis of estradiol 17β, or addition of tamoxifen - an estrogen receptor antagonist, to human placental minces resulted in an increase in the level of LDL-receptor mRNA. This increase could be blocked by the simultaneous addition of estradiol 17β. A concentration dependent effect of estradiol 17β on the level of LDL-receptor mRNA was seen both in first trimester, and term placenta. Addition of human chorionic gonadotropin (hCG) to term placental minces also increased the LDL-receptor mRNA levels. When hCG and cycloheximide were added together, an additive effect was observed. The results obtained in this study suggest that the LDL-receptor mRNA levels in the human placenta are regulated by estradiol 17β and hCG.

LDL-receptor estradiol 17β human chorionic gonadotropin human placenta progesterone synthesis 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Y. Gopi Shanker
    • 1
  • Usha P. Shetty
    • 1
  • A. Jagannadha Rao
    • 1
  1. 1.Departments of Biochemistry and Molecular Reproduction Development and GeneticsIndian Institute of ScienceBangaloreIndia

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