Molecular and Cellular Biochemistry

, Volume 187, Issue 1–2, pp 133–139 | Cite as

Regulation of low density lipoprotein receptor mRNA levels by estradiol 17β and chorionic gonadotropin in human placenta

  • Y. Gopi Shanker
  • Usha P. Shetty
  • A. Jagannadha Rao


Inhibition of synthesis of estradiol 17β by the addition of inhibitors of aromatase, a key enzyme in the biosynthesis of estradiol 17β, or addition of tamoxifen - an estrogen receptor antagonist, to human placental minces resulted in an increase in the level of LDL-receptor mRNA. This increase could be blocked by the simultaneous addition of estradiol 17β. A concentration dependent effect of estradiol 17β on the level of LDL-receptor mRNA was seen both in first trimester, and term placenta. Addition of human chorionic gonadotropin (hCG) to term placental minces also increased the LDL-receptor mRNA levels. When hCG and cycloheximide were added together, an additive effect was observed. The results obtained in this study suggest that the LDL-receptor mRNA levels in the human placenta are regulated by estradiol 17β and hCG.

LDL-receptor estradiol 17β human chorionic gonadotropin human placenta progesterone synthesis 


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  1. 1.
    Albrecht EP, Pepe GJ: Placental steroid hormone biosynthesis in primate pregnancy. Endo Rev 11: 124-150, 1990Google Scholar
  2. 2.
    Csapo AI, Pulkkinen MO, Ruttner B, Sauvage JP, Wiest WG: The significance of human corpus luteum in pregnancy maintenance I: Preliminary studies. Am J Obstet Gynaecol 112: 1061-1069, 1972Google Scholar
  3. 3.
    Van Leusden H, Villee CA: The de novo synthesis of steroids from acetate by preparations of human term placenta. Steroids 6: 31, 1965Google Scholar
  4. 4.
    Gwynne JT, Strauss JF III: The role of lipoproteins in steroidogenesis and cholesterol metabolism in steroidogenic glands. Endo Rev 3: 299, 1982Google Scholar
  5. 5.
    Simpson ER, Billheimer DW, McDonald PC, Roster JC: Uptake and degradation of plasma lipoproteins by human choriocarcinoma cells in culture. Endocrinology 104: 8-17, 1979Google Scholar
  6. 6.
    Simpson ER, McDonald PC: Endocrine physiology of the placenta. Annu Rev Physiol 43: 163-188, 1981Google Scholar
  7. 7.
    Ma PTS, Yamamoto T, Goldstein JL, Brown MS: Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17 α-ethinyl estradiol. Proc Natl Acad Sci USA 83: 792, 1986Google Scholar
  8. 8.
    Henson MC, Babischkin JS, Pepe GJ, Albrecht ED: Effect of the antiestrogen ethamoxytriphetol (MER-25) on placental low density lipoprotein uptake and degradation in baboons. Endocrinology 122: 2019-2026, 1988Google Scholar
  9. 9.
    Grimes RW, Pepe GJ, Albrecht EP: Regulation of human placental trophoblast low-density lipoprotein uptake in vitro by estrogen. J Clin Endocrinol Metab 81: 2675-2679, 1996Google Scholar
  10. 10.
    Gibori G, Chou YD, Khan I, Azhar S, Reaven GM: Regulation of luteal cell lipoprotein receptors, sterol contents and steroidogenesis by estradiol in the pregnant rat. Endocrinology 114: 609-617, 1984Google Scholar
  11. 11.
    Soto E, Silavin SL, Tureck RW, Strauss JF III: Stimulation of progesterone synthesis in luteinized human granulosa cells by human chorionic gonadotropin and 8-bromo 3′,5′ monophosphate: The effect of low density lipoprotein. J Clin Endocrinol Metab 58: 831-837, 1984Google Scholar
  12. 12.
    Veldhuis JD, Azimi P, Garmey J, Juchter D: Follicle stimulating hormone regulation of low density lipoprotein metabolism by swine granulosa cells. Endocrinology 123: 1550-1557, 1988Google Scholar
  13. 13.
    Sharma SC, Usha K. Dighe RR, Rao AJ: Regulation of protein synthesis in the first trimester human placenta by 17β oestradiol and progesterone. Placenta 11: 63-74, 1990Google Scholar
  14. 14.
    Chung CT, Miller RH: A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucl Acid Res 16: 3580, 1988Google Scholar
  15. 15.
    Yamamoto T, Davis CG, Brown MS, Schneider WJ, Casey ML, Goldstein JL, Russel DW: The human LDL receptor: A cysteine rich protein with multiple alu sequences in its mRNA. Cell 139: 27-38, 1984Google Scholar
  16. 16.
    Shetty UP: Studies on the role of estradiol 17β in the regulation of synthesis of proteins by human placenta. Ph.D thesis, Indian Institute of Science, Bangalore, IndiaGoogle Scholar
  17. 17.
    Chomczynski P, Sacchi N: Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159, 1987Google Scholar
  18. 18.
    Thomas PS: Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77: 5201-5205, 1980Google Scholar
  19. 19.
    Sharma SC, Purohit P, Rao AJ: Role of oestradiol 17-β in the regulation of synthesis and secretion of human chorionic gonadotropin by first rimester human placenta. J Mol Endocrinol UK 11: 91-101, 1993Google Scholar
  20. 20.
    Menon KMJ, Jaffe RB: Chorionic gonadotropin sensitive adenylate cyclase in human placenta. J Clin Endocrinol Metab 36: 1104-1109, 1973Google Scholar
  21. 21.
    Golos TG, Strauss JF III: Regulation of low density lipoprotein receptor gene expression in cultured human granulosa cells: Roles of hCG, 8-bromo 3′,5′ cyclic adenosine monophosphate and protein synthesis. Mol Endocrinol 1: 321-326, 1987Google Scholar
  22. 22.
    Goldstein JL, Brown MS: The low density lipoprotein pathway and its relation to atherosclerosis. Annu Rev Biochem 46: 897-930, 1977Google Scholar
  23. 23.
    Furuhashi M, Seo H, Mizutari S, Nariota O, Tomoda Y, Matsui N: Expression of low density lipoprotein receptor gene in human placenta during pregnancy. Mol Endocrinol 31: 1252-1256, 1989Google Scholar
  24. 24.
    Chakraborty J, Hopkins R, Parke DV: Inhibition studies on the aromatization of androst-4-ene-3, 17-dione by human placental microsomal preparations. Biochem J 130: 19P-20P, 1972Google Scholar
  25. 25.
    Golos TG, Strauss JF III: Regulation of low density lipoprotein receptor synthesis in cultured leuteinized human granulosa cells by human chorionic gonadotropin and 8 bromo-cAMP. J Biol Chem 260: 14399-14402, 1985Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Y. Gopi Shanker
    • 1
  • Usha P. Shetty
    • 1
  • A. Jagannadha Rao
    • 1
  1. 1.Departments of Biochemistry and Molecular Reproduction Development and GeneticsIndian Institute of ScienceBangaloreIndia

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