Molecular and Cellular Biochemistry

, Volume 170, Issue 1–2, pp 43–51 | Cite as

Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells

  • Antonio D. McCarthy
  • Susana B. Etcheverry
  • Liliana Bruzzone
  • Ana M. Cortizo


Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner.

advanced glycation endproducts diabetes mellitus osteoblastic cells cell proliferation cell differentiation bone 


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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Antonio D. McCarthy
    • 1
  • Susana B. Etcheverry
    • 1
  • Liliana Bruzzone
    • 2
  • Ana M. Cortizo
    • 1
  1. 1.Cátedra de Bioquímica Patólogica, Facultad de Ciencias ExactasUniversidad Nacional de La PlataLa PlataArgentina
  2. 2.División Química Analítica, Facultad de Ciencias ExactasUniversidad Nacional de La PlataLa PlataArgentina

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