Molecular and Cellular Biochemistry

, Volume 178, Issue 1–2, pp 377–382

Anabolic effect of genistein and genistin on bone metabolism in the femoral-metaphyseal tissues of elderly rats: The genistein effect is enhanced by zinc

  • Masayoshi Yamaguchi
  • Ying Hua Gao
Article

DOI: 10.1023/A:1006809031836

Cite this article as:
Yamaguchi, M. & Hua Gao, Y. Mol Cell Biochem (1998) 178: 377. doi:10.1023/A:1006809031836

Abstract

The effect of genistein and genistin on bone components in the femoral-metaphyseal tissues obtained from elderly female rats was investigated in vitro. The metaphyseal tissues were cultured for 24 h in a medium containing either vehicle, genistein (10-8-10-5 M) or genistin (10-7-10-5 M). The presence of genistein or genistin caused a significant increase in alkaline phosphatase activity, deoxyribonucleic acid (DNA) and calcium contents in the metaphyseal tissues. The effect of genistein was greater than that of genistin. The bone components increased by genistein (10-5 M) or genistin (10-5 M) were completely blocked by the presence of cycloheximide (10-6 M). The presence of zinc sulfate (10-5 M) caused a significant increase in the genistein (10-5 M)-elevated alkaline phosphatase activity, DNA and calcium contents. The enhancement with zinc was not seen by genistin (10-5 M). The stimulatory effect of zinc on the genistein-induced increase in bone components of the metaphyseal tissues was completely blocked by the presence of cycloheximide (10-6 M). The present results suggest that genistein and genistin have an anabolic effect on bone metabolism in the femoral-metaphyseal tissues of elderly rats, and that the genistein effect is enhanced by zinc, an essential trace element.

bone metabolism genistein genistin zinc femoral metaphysics aged rats 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Masayoshi Yamaguchi
    • 1
  • Ying Hua Gao
    • 1
  1. 1.Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional SciencesUniversity of ShizuokaShizuoka CityJapan

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