Clinical & Experimental Metastasis

, Volume 17, Issue 2, pp 177–181

Experimental metastasis is suppressed in MMP-9-deficient mice

  • Takeshi Itoh
  • Masatoshi Tanioka
  • Hidetoshi Matsuda
  • Hirofumi Nishimoto
  • Takayuki Yoshioka
  • Ryuji Suzuki
  • Masahiro Uehira
Article

Abstract

Matrix metalloproteinases (MMPs) are thought to play a key role in tumor invasion and metastasis. The role of MMP-9 (gelatinase B) in tumor metastasis was examined in MMP-9-deficient mice produced by gene targeting using embryonic stem cells. MMP-9-deficient mice develop normally and are fertile. In these mice, the number of metastatic colonies of B16-BL6 melanoma cells or Lewis lung carcinoma cells that were implanted intravenously fell by 45% for B16-BL6 melanoma and 59% for Lewis lung carcinoma (p=0.03 and p=0.0043, respectively). Gelatin zymography showed that both tumor cell lines did not secrete MMP-9 by themselves but the host cells surrounding the tumor cells secrete MMP-9 in vivo. These results indicated that host-derived MMP-9 plays an important role in the process of tumor metastasis.

experimental tumor metastasis gelatinase knockout mice matrix metalloproteinase 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Takeshi Itoh
    • 1
  • Masatoshi Tanioka
    • 1
  • Hidetoshi Matsuda
    • 1
  • Hirofumi Nishimoto
    • 1
  • Takayuki Yoshioka
    • 2
  • Ryuji Suzuki
    • 1
  • Masahiro Uehira
    • 1
  1. 1.Shionogi & Co., LtdShionogi Institute for Medical ScienceJapan
  2. 2.Discovery Research LaboratoriesShionogi & Co., LtdFukushima-ku, OsakaJapan

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