Plant Cell, Tissue and Organ Culture

, Volume 62, Issue 3, pp 169–173 | Cite as

Thidiazuron-induced de novo shoot organogenesis on seedlings, etiolated hypocotyls and stem segments of Huang-qin

  • H. Li
  • S. J. Murch
  • P. K. Saxena


Development of an efficient in vitro propagation system for Huang-qin (Scutellaria baicalensis), a traditional Chinese medicinal plant used in the treatment of a wide range of human ailments, is described. Thidiazuron [TDZ: N-phenyl-N′- (1,2,3-thidiazol-5-ylurea)] effectively induced regeneration on cultured intact seedlings, etiolated hypocotyl explants and sterile stem segments of Huang-qin. Histological examinations of excised hypocotyl or nodal explants revealed that adventitious shoots formed through an intermediate callus. Comparison of TDZ-induced regeneration in the three tissue types indicated that isolation of explants was not essential for optimal regenerative efficiency. Significantly more regenerants formed along hypocotyls of intact seedlings (20 shoots/explant) than were observed on excised hypocotyls (9.7 shoots/explant) indicating that endogenous metabolites produced in adjacent tissues provided resources for the shoot initiation. More than 95% of de novo regenerants formed roots and then intact plantlets under either sterile culture or greenhouse conditions. Regeneration protocols developed in this study may provide the basis for improvement of this crop through the identification of medicinally active constituents and eventual development optimized pharmaceutical products.

thidiazuron organogenesis Huang-qin Scutellaria baicalensis phytopharmaceutical 


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  1. Betz W (1998) Epidemic of renal failure due to herbals. Sci. Rev. Alt. Med. 2: 12–13Google Scholar
  2. Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158PubMedCrossRefGoogle Scholar
  3. Gao D, Tawa R, Masaki H, Okano Y & Sakurai H (1998) Protective effects of baicalein against cell damage by reactive oxygen species. Chem. Pharm. Bull. 46: 383–1387Google Scholar
  4. Johansen DA (1940) Plant Microtechnique. McGraw-Hill Inc., New York, USAGoogle Scholar
  5. Kyo R, Nakahata N, Sakakibara I, Kubo M & Ohizumi Y (1998) Baicalin and baicalein, constituents of an important medicinal plant, inhibit intracellular Ca2+ elevation by reducing phospholipase C activity in C6 rat glioma cells. J. Pharm. Pharmacol. 50: 1179–1182PubMedGoogle Scholar
  6. Malik KA & Saxena PK (1992) In vitro regeneration of plants: A novel approach. Naturwissenschaften 79: 136–137CrossRefGoogle Scholar
  7. Mok MC & Mok DWS (1985) The metabolism of (14C)-thidiazuron in callus cultures of Phaseolus lunatus. Physiol. Plant. 65: 427–432CrossRefGoogle Scholar
  8. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  9. Murch SJ, Simmons CB & Saxena PK (1997) Melatonin in feverfew and other medicinal plants. Lancet. 350: 1598–1599PubMedCrossRefGoogle Scholar
  10. Murch SJ, Choffe KL, Victor JMR, Slimmon TY, KrishnaRaj S & Saxena PK (1999) Thidiazuron-induced regeneration from hypocotyl cultures of St. John's wort (Hypericum perforatum cv. Anthos). Plant Cell Rep. (In press)Google Scholar
  11. Murthy BNS, Murch SJ & Saxena PK (1995) Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): Endogenous growth regulator levels and significance of cotyledons. Physiol. Plant. 94: 268–276CrossRefGoogle Scholar
  12. Murthy BNS, Murch SJ & Saxena PK (1998) Thidiazuron: A potent regulator of in vitro plant morphogenesis. In vitro Cell. Dev. Biol. 34: 267–275CrossRefGoogle Scholar
  13. Skoog F & Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. 11: 118–140Google Scholar
  14. Steward FC, Mapes MO, Kent AE & Holsten RD (1964) Growth and development of cultured plant cells. Science 143: 20–27PubMedGoogle Scholar
  15. Zhou Ruzheng & Gu G (1991) Pharmaceutical study of Huang-qin. Chinese Medicine 13: 28–29Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  1. 1.Department of Plant AgricultureUniversity of GuelphGuelph, OntarioCanada

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