Thidiazuron-induced de novo shoot organogenesis on seedlings, etiolated hypocotyls and stem segments of Huang-qin
- 137 Downloads
Development of an efficient in vitro propagation system for Huang-qin (Scutellaria baicalensis), a traditional Chinese medicinal plant used in the treatment of a wide range of human ailments, is described. Thidiazuron [TDZ: N-phenyl-N′- (1,2,3-thidiazol-5-ylurea)] effectively induced regeneration on cultured intact seedlings, etiolated hypocotyl explants and sterile stem segments of Huang-qin. Histological examinations of excised hypocotyl or nodal explants revealed that adventitious shoots formed through an intermediate callus. Comparison of TDZ-induced regeneration in the three tissue types indicated that isolation of explants was not essential for optimal regenerative efficiency. Significantly more regenerants formed along hypocotyls of intact seedlings (20 shoots/explant) than were observed on excised hypocotyls (9.7 shoots/explant) indicating that endogenous metabolites produced in adjacent tissues provided resources for the shoot initiation. More than 95% of de novo regenerants formed roots and then intact plantlets under either sterile culture or greenhouse conditions. Regeneration protocols developed in this study may provide the basis for improvement of this crop through the identification of medicinally active constituents and eventual development optimized pharmaceutical products.
Unable to display preview. Download preview PDF.
- Betz W (1998) Epidemic of renal failure due to herbals. Sci. Rev. Alt. Med. 2: 12–13Google Scholar
- Gao D, Tawa R, Masaki H, Okano Y & Sakurai H (1998) Protective effects of baicalein against cell damage by reactive oxygen species. Chem. Pharm. Bull. 46: 383–1387Google Scholar
- Johansen DA (1940) Plant Microtechnique. McGraw-Hill Inc., New York, USAGoogle Scholar
- Murch SJ, Choffe KL, Victor JMR, Slimmon TY, KrishnaRaj S & Saxena PK (1999) Thidiazuron-induced regeneration from hypocotyl cultures of St. John's wort (Hypericum perforatum cv. Anthos). Plant Cell Rep. (In press)Google Scholar
- Skoog F & Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. 11: 118–140Google Scholar
- Zhou Ruzheng & Gu G (1991) Pharmaceutical study of Huang-qin. Chinese Medicine 13: 28–29Google Scholar