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Plant Molecular Biology

, Volume 43, Issue 4, pp 503–513 | Cite as

Characterisation of complementary DNAs from the expressed sequence tag analysis of life cycle stages of Laminaria digitata (Phaeophyceae)

  • Florent Crépineau
  • Thomas Roscoe
  • Raymond Kaas
  • Bernard Kloareg
  • Catherine Boyen
Article

Abstract

Laminariales (Phaeophyceae, Heterokonta) are characterised by a heteromorphic digenetic life cycle with a filamentous, microscopic gametophyte and a highly evolved, macroscopic sporophyte. With the ultimate goal of comparing gene expression in each life cycle stage, complementary DNA libraries were constructed from sporophytes and gametophytes of Laminaria digitata. A set of ca. 500 expressed sequence tags (EST) was generated from each life history phase, by single-run partial sequencing of randomly picked cDNA clones. Comparison of the EST deduced amino acid sequences with database protein sequences assigned a putative identity for 39% of the 412 gametophyte clones and 48% of the 493 sporophyte clones sequenced thus far. These data represent more than 152 different proteins now probably identified in L. digitata. Several of those newly identified proteins are of interest to our understanding of the molecular physiology of kelps, for example their carbon-concentrating mechanisms, cell wall biosynthesis and halogen metabolism. EST analysis also confirmed that genes with long 3′-UTRs are widespread in Laminariales and the study of 5′-UTRs allowed the identification of a Kozak consensus sequence, c(A/C)A(A/C)CAUGGc(G/T). Several potential developmentally regulated differences in gene expression are discussed.

ACEDB EST Kozak sequence Laminaria digitata 3′-UTR 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Florent Crépineau
    • 1
  • Thomas Roscoe
    • 2
  • Raymond Kaas
    • 3
  • Bernard Kloareg
    • 1
  • Catherine Boyen
    • 1
  1. 1.Laboratoires Goëmar, Observatoire Océanologique de RoscoffUMR 1931, CNRSRoscoff cedexFrance
  2. 2.Physiologie et Biologie Moléculaire des PlantesCNRS, UMR 5545Perpignan cedexFrance
  3. 3.Physiologie de la Reproduction des MacroalguesIFREMER, Centre de NantesNantes cedex 03France

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