Breast Cancer Research and Treatment

, Volume 61, Issue 2, pp 139–143

Pilot studies on the p53 gene in nipple aspirate fluid from patients with breast cancer

  • Hamish A. Phillips
  • Grahame C.W. Howard
  • William R. Miller
Article

DOI: 10.1023/A:1006487315587

Cite this article as:
Phillips, H.A., Howard, G.C. & Miller, W.R. Breast Cancer Res Treat (2000) 61: 139. doi:10.1023/A:1006487315587

Abstract

Nipple Aspirate Fluid (NAF) from patients with breast cancer is a potential source of exfoliated tumour material amenable to molecular biological study, but few such data have been reported. In this study we demonstrate that polymerase chain reaction (PCR) amplification of p53 gene DNA is achievable in a proportion of NAF samples from breast cancer patients. Subsequently four NAF samples from patients whose primary tumours were identified as having a defined p53 mutation were studied by single stranded conformational polymorphism analysis (SSCP). Two samples yielded PCR product indistinguishable from wild type and two yielded no product. Whilst no cancer-related genetic mutations were demonstrated in NAF samples, further study is warranted.

breast cancer mutation nipple aspirate fluid p53 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Hamish A. Phillips
    • 1
    • 2
  • Grahame C.W. Howard
    • 1
    • 2
  • William R. Miller
    • 2
  1. 1.NHS Department of Clinical OncologyWestern General HospitalEdinburghUK
  2. 2.Department of Oncology, Western General HospitalUniversity of EdinburghEdinburghUK

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