Plant Molecular Biology

, Volume 45, Issue 3, pp 307–315 | Cite as

The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide sequence and gene organization

  • Christian Schmitz-Linneweber
  • Rainer M. Maier
  • Jean-Pierre Alcaraz
  • Annick Cottet
  • Reinhold G. Herrmann
  • Regis Mache


The chloroplast chromosome of spinach (Spinacia oleracea) is a double-stranded circular DNA molecule of 150 725 nucleotide pairs. A comparison of this chromosome with those of the three other autotrophic dicotyledons for which complete DNA sequences of plastid chromosomes are available confirms a conserved overall structure. Three classes of open reading frames were distinguished: (1) genes of known function which include 108 unique loci, (2) three hypothetical chloroplast reading frames (ycfs) that are highly conserved interspecifically, and (3) species-specific or rapidly diverging 'open reading frames'. A detailed transcript study of one of the latter (ycf15) shows that these loci may be transcribed, but do not constitute protein-coding genes.

chloroplast open reading frames plastid chromosome spinach Spinacia oleracea,ycf15 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Christian Schmitz-Linneweber
    • 1
  • Rainer M. Maier
    • 1
  • Jean-Pierre Alcaraz
    • 2
  • Annick Cottet
    • 2
  • Reinhold G. Herrmann
    • 1
  • Regis Mache
    • 2
  1. 1.Botanisches Institut der Ludwig-Maximilians-UniversitätMünchenGermany
  2. 2.Laboratoire de Génétique Moléculaire des PlantesUniversité Joseph Fourier and CNRSGrenobleFrance

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