Plant Cell, Tissue and Organ Culture

, Volume 61, Issue 1, pp 29–35 | Cite as

Efficient rooting for establishment of papaya plantlets by micropropagation

  • Tsong-Ann Yu
  • Shyi-Dong Yeh
  • Ying-Huey Cheng
  • Jiu-Sherng Yang


A low cost micropropagation protocol to produce high quality root systems which are easy and economical to acclimatize is essential for large-scale micropropagation of papaya (Carica papaya L.). In this study, individual shoots (>0.5 cm) with 23 leaves from in vitro papaya multiple shoots were cultured on MS agar medium containing 2.5 μM IBA under dark conditions for 1 week for root induction. They were then transferred to agar or vermiculite media, containing half strength MS medium, under aerated or non-aerated conditions, for root development. Rooting percentage of shoots cultured for 2 weeks in aerated vermiculite was 94.5%, compared with 90.0% in non-aerated vermiculite, 71.1% in aerated agar, and 62.2% in non-aerated agar. Shoots with roots were acclimated in vermiculite under 100% RH for 1 week and then under ambient conditions for 2 weeks in a temperature-controlled growth chamber (28 °C). The survival rates of the plantlets were 94.5% from aerated vermiculite, 87.8% from non-aerated vermiculite, 42.2% from aerated agar, and 35.6% from non-aerated agar. Thus, root induction in low-concentration IBA agar medium followed by root development in vermiculite containing half strength MS medium under aerated conditions results in efficient rooting of in vitro papaya shoots.

aeration agar papaya rooting plantlet survival vermiculite 


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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Tsong-Ann Yu
    • 1
  • Shyi-Dong Yeh
    • 2
  • Ying-Huey Cheng
    • 2
  • Jiu-Sherng Yang
    • 1
  1. 1.Department of BotanyNational Chung-Hsing UniversityTaichungTaiwan, Republic of China
  2. 2.Department of Plant PathologyNational Chung-Hsing UniversityTaichungTaiwan, Republic of China

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