Plant Molecular Biology

, Volume 42, Issue 6, pp 899–913

Cloning, mapping and expression analysis of barley MADS-box genes

  • Jürgen Schmitz
  • Rainer Franzen
  • Thi Ha Ngyuen
  • Federico Garcia-Maroto
  • Carlo Pozzi
  • Francesco Salamini
  • Wolfgang Rohde
Article

Abstract

Six MADS-box cDNA clones were isolated by heterologous screening from a barley inflorescence cDNA library. Based on sequence comparison to known MADS-box genes, the barley MADS-box (BM) genes were grouped into three distinct phylogenetic subclasses of the MADS-box gene family. The three MADS-box genes BM3, BM5 and BM8 share similarities with genes of the SQUAMOSA (SQUA) subgroup, while BM7 and BM9 belong to the AGAMOUS-LIKE 2 (AGL2) subgroup. BM1 resembles MADS-box genes described as solitary sequences or orphan genes. Expression analysis of the barley MADS-box genes revealed expression patterns that are not characteristic of the barley MADS-box genes of the SQUA subgroup, while expression of BM7 and BM9 was largely as expected for the AGL2 subgroup. BM1 is mainly expressed in vegetative tissues and its primary transcript undergoes alternative splicing such that the corresponding mRNAs differ by two codons. The genes BM1, BM3 and BM8 were mapped by analysis of single-nucleotide polymorphisms onto barley chromosomes 4, 2 and 7, respectively.

alternative splicing flower development homeotic genes Hordeum vulgare mutants 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Jürgen Schmitz
    • 1
  • Rainer Franzen
    • 1
  • Thi Ha Ngyuen
    • 1
  • Federico Garcia-Maroto
    • 2
  • Carlo Pozzi
    • 1
  • Francesco Salamini
    • 1
  • Wolfgang Rohde
    • 1
  1. 1.MPI für ZüchtungsforschungKölnGermany
  2. 2.Departamento de BioquimicaUniversidad de Almeria, La Canada de San UrbanoAlmeriaSpain

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