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Recovery and regeneration of embryogenic cultures from female flowers of False Horn Plantain

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Abstract

Somatic embryogenesis from immature male flowers in Musa is only suitable for genotypes with a male bud. Six friable embryogenic cultures were obtained from 28 cultured buds of female flowers of the AAB False Horn Plantains, ‘Curraré’ and ‘Curraré Enano’. Embryogenic suspensions were established from these embryogenic cultures. Somatic embryogenesis was demonstrated histologicaly. Regeneration of plants was obtained either from somatic embryos directly isolated from embryogenic cultures or from suspensions after plating on a semi-solid medium. This study demonstrates that somatic embryogenesis from immature flowers is suitable for genotypes of Musa with or without male buds.

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Authors and Affiliations

  1. CATIE, Centro Agronómico Tropical de Investigación y Enseñanza, Unidad de Biotecnología, 7170 Turrialba, Costa Rica

    A. Grapin, J-L. Ortíz, T. Lescot & F.X. Côte

  2. CIRAD, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, BP 5035, 34032, Montpellier Cedex 1, France

    A. Grapin, T. Lescot, N. Ferrière & F.X. Côte

Authors
  1. A. Grapin
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  2. J-L. Ortíz
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  3. T. Lescot
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  4. N. Ferrière
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  5. F.X. Côte
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Correspondence to F.X. Côte.

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Grapin, A., Ortíz, JL., Lescot, T. et al. Recovery and regeneration of embryogenic cultures from female flowers of False Horn Plantain. Plant Cell, Tissue and Organ Culture 61, 237–244 (2000). https://doi.org/10.1023/A:1006423304033

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  • DOI: https://doi.org/10.1023/A:1006423304033

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