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Veterinary Research Communications

, Volume 24, Issue 6, pp 379–387 | Cite as

In Vitro Metabolism of Fumonisin B1 by Ruminal Microflora

  • F. Caloni
  • M. Spotti
  • H. Auerbach
  • H. Op den Camp
  • J. Fink Gremmels
  • G. Pompa
Article

Abstract

Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium moniliforme and F. proliferatum. Little is known of its metabolic fate after oral ingestion in ruminants, but these animals are reported to be tolerant towards FB1. The metabolism of this mycotoxin was evaluated following incubation (1 μg/ml) in ruminal fluid for up to 72 h, in the presence or absence of alfalfa as a substrate for microbial growth, using a model rumen (sealed flask, anaerobic conditions, exclusion of light, gentle agitation, 39°C). The decrease in FB1 concentration and the production of short-chain fatty acids were determined. FB1 had no effect on SCFA production. After 72 h incubation, FB1 depletion was 12% and 18% in samples with and without alfalfa, respectively. No hydrolysed metabolites (aminopolyols or aminopentol) were detected. These results indicate that FB1 is poorly metabolized in the rumen and suggest that such metabolism is not the cause of the tolerance to this toxin displayed by ruminants.

biodegradation fumonisin B1 metabolites microflora mycotoxin rumen short-chain fatty acid 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • F. Caloni
    • 1
  • M. Spotti
    • 1
  • H. Auerbach
    • 2
  • H. Op den Camp
    • 3
  • J. Fink Gremmels
    • 2
  • G. Pompa
    • 1
  1. 1.Facoltà di Medicina VeterinariaIstituto di Farmacologia e Tossicologia VeterinariaMilanItaly
  2. 2.Department of Basic Science, Division of Pharmacy, Pharmacology and ToxicologyUtrecht
  3. 3.Netherlands

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