Rapid propagation of Eleutherococcus senticosus via direct somatic embryogenesis from explants of seedlings
- 187 Downloads
Explants from three different parts (cotyledon, hypocotyl or root) of one week-old seedlings of Eleutherococcus senticosus were cultured on Murashige and Skoog (MS) medium with 1.0 mg l-1 2,4-D. Somatic embryos were formed directly from the surfaces of explants. The frequency of direct somatic embryo formation was the highest in the hypocotyl segments (75%) as compared to cotyledon (56%) or root segments (12%). When hypocotyl explants from 3 different stages of seedlings (zero, one or three week-old) were cultured on MS medium with 1.0 mg l-1 2,4-D, the frequency of somatic embryo formation rapidly declined as the zygotic embryos germinated. However most somatic embryos (93%) from explants of zygotic embryos developed as fused state (multiple embryo), whereas somatic embryos (over 89%) from more developed seedlings developed into single state (single embryo). Single embryos germinated and regenerated into plantlets with both shoots and roots, while multiple embryos only regenerated into only multiple shoots. Plantlets that regenerated from single embryos of E. senticosus were acclimatized in a greenhouse.
Unable to display preview. Download preview PDF.
- Brekhman II (1960) A new medicinal plant of the family Araliceae the spiny Eleutherococcus. Izv. Sibir. Otdel., Akad. Nauk. USSR 9: 113–120Google Scholar
- Choi YE & Soh WY(1994) Origin of somatic embryos from excised cotyledons of ginseng.J. Plant Biol. 37: 365–370Google Scholar
- Gui Y, Guo Z, Ke S & Skirvin RH (1991) Somatic embryogenesis and plant regeneration in Acanthopanax senticosus. Plant Cell Rep. 49: 514–516Google Scholar
- Isoda S & Shoji J (1994) Studies on the cultivation of Eleutherococcus senticosus Maxim. II. On the germination and raising of seedling. Nat. Med. 48: 75–81Google Scholar
- Lee WT (1979) Distribution of Acanthopanax plants in Korea. Korean J. Pharmocol. 10: 103–107Google Scholar
- Park HK (1997) Germination morphology and growth characteristics of Kaciogaphi (Eleutherococcus senticosus). Ph.D. thesis, Chonbuk National University, Chonju, South KoreaGoogle Scholar
- Pence VC, Hasegawa PM & Janick J (1980) Initiation and development of asexual embryos of Theobroma cacao L. in vitro. Z. Pflanzenphysiol. 98: 1–14Google Scholar