Plant Growth Regulation

, Volume 31, Issue 3, pp 215–224 | Cite as

Relationship between polyamines and paraquat toxicity in sunflower leaf discs

  • María P. Benavides
  • Susana M. Gallego
  • María E. Comba
  • María L. Tomaro
Article

Abstract

Polyamines have been reported as efficient antioxidantcompounds in plants. Sunflower leaf discs, treatedwith 100 µM paraquat (PQ), a well known oxidativestress inducer, showed decreased levels of putrescine(Put), spermidine (Spd) and spermine (Spm) (between33% and 80% with respect to the controls). Argininedecarboxylase (ADC) and ornithine decarboxylase (ODC)activities decreased 42% and 33% respectively. Amongthe markers of oxidative stress measured after PQtreatment, chlorophyll and glutathione content werereduced (30% and 49% respectively) andthiobarbituric acid reactive substances (TBARS)content increased (60%). Superoxide dismutase (SOD)activity declined 60% with respect to the control andlipoxygenase (LOX) increased 25% when leaf-discs weretreated with the herbicide. Pretreatment withexogenous polyamines (1 mM) reversed paraquat toxicityto different degrees according to the polyamine and/orthe tested parameter. Spermidine was able to inhibitchlorophyll loss, while Spm reverted the effect of PQon the level of TBARS almost completely and alsorestored SOD activity close to control values.Putrescine was the least effective as an oxidantprotectant. These results provide support for theargument that polyamines are effective antioxidantsthrough their ability to act as radical scavengers.

antioxidants Helianthus annuus L. paraquat polyamines 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anderson ME (1985) Determination of glutathione and glutathione disulfide in biological samples. Methods in Enzymol 113: 545-548Google Scholar
  2. 2.
    Aziz A and Larher F (1996) Changes in polyamine titers associated with the proline response and osmotic adjustment of rape leaf discs submitted to osmotic stress. Plant Sci 112: 175-186Google Scholar
  3. 3.
    Bagni N and Torrigiani P (1992) Polyamines: a new class of growth susbtances. In: Karssen CM, van Loon LC and Vreugdenhil D (eds) Progress in Plant Growth Regulation. Dordrecht: Kluwer Academic Publishers, pp 264-275Google Scholar
  4. 4.
    Becana M, Aparicio-Tejo P, Irigoyen JJ and Sánchez-Díaz M (1986) Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa. Plant Physiology 82: 1169-1171Google Scholar
  5. 5.
    Benavides MP, Aizencang G and Tomaro ML (1997) Polyamines in Helianthus annuus L. during germination under salt stress. J Plant Growth Regul 16: 205-211Google Scholar
  6. 6.
    Besford RT, Richardson CM, Campos JL and Tiburcio AF (1993) Effect of polyamines on the stabilization of molecular complexes in thylakoid membranes of osmotically-stressed oat leaves. Planta 189: 201-206Google Scholar
  7. 7.
    Borrel A, Carbonell L, Farras R, Puig-Parellada P and Tiburcio AF (1997) Polyamines inhibited lipid peroxidation in senescing oat leaves. Physiol Plant 99: 385-390Google Scholar
  8. 8.
    Bors W, Langebartels C, Michel C and Sandermann H (1989) Polyamines as radical scavengers and protectans against ozone damage. Phytochemistry 28: 1589-1595Google Scholar
  9. 9.
    Bradford MM (1956) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254Google Scholar
  10. 10.
    Bratton DL (1994) Polyamine inhibition of transbilayer movement of plasma membrane phospholipids in the erythrocite ghost. J Biol Chem 269: 22517-22523Google Scholar
  11. 11.
    Calderbank A (1968) The bipyrydinium herbicides. Adv Pest Cont Res 8: 127-235Google Scholar
  12. 12.
    Chang CJ and Kao CH (1997) Paraquat toxicity is reduced by polyamines in rice leaves. Plant Growth Regul 22: 163-168Google Scholar
  13. 13.
    Drolet G, Dumbroff EB, Legge RL and Thompson JE (1986) Radical scavenging properties of polyamines. Phytochemistry 25: 367-371Google Scholar
  14. 14.
    Evans RT and Malmberg RL (1989) Do polyamines have roles in plant development? Ann Rev Plant Physiol Plant Mol Biol 40: 235-269Google Scholar
  15. 15.
    Flores HE and Galston AW (1984) Osmotic stress induced polyamine accumulation in cereal leaves. I. Physiological parameters of the response. Plant Physiol 75: 102-109Google Scholar
  16. 16.
    Flores HE (1991) Changes in polyamine metabolism in response to abiotic stress. In: Slocum RD and Flores HE (eds) Biochemistry and Physiology of Polyamines in Plants. Boca Raton, Florida: CRC Press, pp 213-227Google Scholar
  17. 17.
    Foyer CH, Descourvieres P and Kunert KJ (1994) Protection against oxygen radicals: important defense mechanism studied in transgenic plants. Plant Cell Environ 17: 507-523Google Scholar
  18. 18.
    Galston AW and Kaur-Sawhney R (1995) Polyamines as endogenous plant growth regulators. In: Davies PJ (ed) Plant Hormones: Physiology, Biochemistry and Molecular Biology, 2nd De. Dordrecht: Kluwer Academic Publishers, pp 158-178Google Scholar
  19. 19.
    Heath RL and Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125: 189-198Google Scholar
  20. 20.
    Hoagland DR and Arnon DI (1957) California Agriculture Experiment Station. Circular 347Google Scholar
  21. 21.
    Kitada M, Igarashi K, Hirose S and Kitagawa K (1979) Inhibition by polyamines of lipid peroxide formation in rat liver microsomes. Biochem Biophys Res Com 87: 388-394Google Scholar
  22. 22.
    Kramer GF, Norman HA, Krizek DT and Mirecki RM (1991) Influence of UV radiation on polyamines, lipid peroxidation and membrane lipids in cucumber. Phytochemistry 30: 2101-2108Google Scholar
  23. 23.
    Kurepa J, Smalle J, van Montagu M and Inzé D (1998) Polyamines and paraquat toxicity in Arabidopsis thaliana. Plant Cell Physiol 39(9): 987-992Google Scholar
  24. 24.
    Langebartels C, Kerner K, Leonardi S, Schraudner M, Trost M, Heller W and Sandermann H Jr (1991) Biochemical plant responses to ozone. Differential induction of polyamine and ethylene biosynthesis in tobacco. Plant Physiol 95: 882-889Google Scholar
  25. 25.
    Lupu R, Grossman S and Cohen Y (1980) The involvement of lipoxygenase and antioxidants in pathogenesis of powdery mildew on tobacco plants. Physiol Plant Pathol 16: 241-248Google Scholar
  26. 26.
    Noctor G, Arisi AM, Jouanin L, Kunert KJ, Rennenberg H and Foyer C (1998) Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants. J Exp Bot 49: 623-647Google Scholar
  27. 27.
    Schupp R and Rennenberg H (1988) Diurnal changes in the glutathione content of spruce needles (Picea abies L.). Plant Science 57: 113-117Google Scholar
  28. 28.
    Seiler N, Delcros JG and Moulinoux JP (1996) Polyamine transport in mammalian cells. An update. Int J Biochem Cell Biol 28: 843-861Google Scholar
  29. 29.
    Seiler N and Dezeure F (1990) Polyamine transport in mammalian cells. Int J Biochem 22: 211-218Google Scholar
  30. 30.
    Smith BN and Meeuse BJD (1966) Production of volatiles amines in some Arum lily species. Plant Physiol 41: 343-347Google Scholar
  31. 31.
    Tadolini B, Cabrini L, Landi L, Varani E and Pasquali P (1984) Polyamine binding to phospolipid vesicles and inhibition of lipid peroxidation. Biochem Biophys Res Com 122: 550-555Google Scholar
  32. 32.
    Tadolini B (1988) Polaymine inhibition of lipoperoxidation. The influence of polyamines on iron oxidation in the presence of compounds mimicking phospholipids polar heads. Biochem J 249: 33-36Google Scholar
  33. 33.
    Tiburcio AF, Besford RT, Capell T, Borrell A, Testillano PS and Risueño MC (1994) Mechanisms of polyamine action during senescence responses induced by osmotic stress. J Exp Bot 45: 1789-1800Google Scholar
  34. 34.
    Tiburcio AF, Altabella T, Borrel T and Masgrau C (1997). Polyamines metabolism and its regulation. Physiol Plant 100: 664-674Google Scholar
  35. 35.
    Ye B, Muller HH, Zhang J and Gressel J (1997) Constitutively elevated levels of putrescine and putrescine generating enzymes correlated with oxidant stress resistance in Conyza bonariensis and wheat. Plant Physiol 115: 1443-1451Google Scholar
  36. 36.
    Wintermans JF and De Mots A (1965) Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol. Biochim Biophys Acta 109: 448-453Google Scholar
  37. 37.
    Zheleva D, Tsonev T, Sergiev Y and Karanov E (1994) Protective effect of exogenous polyamines against atrazine in pea plants. J Plant Growth Regul 13: 203-211Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • María P. Benavides
    • 1
  • Susana M. Gallego
    • 1
  • María E. Comba
    • 1
  • María L. Tomaro
    • 1
  1. 1.Cátedra de Química Biológica Vegetal, Departamento de Química Biológica, Facultad de Farmacia y BioquímicaUniversidad de Buenos AiresBuenos AiresArgentina

Personalised recommendations