Abstract
Jojoba (Simmondsia chinensis, (Link) Schneider) seedling explants were cultured on a modified Driver Kuniyuki medium, supplemented with various concentrations of 6-benzyladenine alone and in combination with silver nitrate. Shoot proliferation was successful at all the concentrations tested, with a maximum number of 15.2 shoots per original explant. Shoots produced during the proliferation stage were treated with α-naphthaleneacetic acid, indole-3-butyric acid and indole-3-acetic acid to induce rhizogenesis, reaching 64% rooting in some treatments. When the rooted explants were transferred to the mist system for acclimatization, 90% of them survived and continued to grow after a period of one month.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alcaraz ML & Ayla-Rocha B (1982) Asexual reproduction of Jojoba by layering. HortScience 17: 893
Babic V & Nescovic M (1984) Propagation of three blackberry cultivars from small apical buds in vitro. J. Hort. Sci. 59: 183–185
Benzioni A, Nerd A, Rosengartner Y & Mills D (1992) The effect of NaCl salinity on growth and development of Jojoba clones. I. Young plants. J. Plant Physiol. 139: 731–736
Chaturvedi HC & Sharma M (1989) In vitro production of cloned plants of Jojoba (Simmondsia chinensis (Link) Schneider) through shoot proliferation in long term culture. Plant Sci. 63: 199–207
Collet GF (1988) Improvement to induce rooting of fruit trees in vitro. Acta Hortic. 227: 318–324
Driver JA & Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. HortScience 19: 507–509
Faria JLC & Segura J (1997) In vitro control of adventitious bud differentiation by inorganic medium compounds and silver thiosulphate in explants of Passiflora edulis var flavicarpa. In Vitro Cell. Dev. Biol. Plant 33: 209–212
Franklin CI & Dixon RA (1994) Initiation and maintenance of callus and cell suspension cultures. In: Dixon RA & Gonzales RA (eds) Plant Cell Culture. A Practical Approach (2nd edn) (pp 1–25). Oxford University Press, New York
George EF (1993) Plant propagation by tissue culture. Part 1, The Technology. Exegetics Ltd, Edington, Wilts, England
Gill MIS, Singh Z, Dhillon BS & Gosal SS (1994) Somatic embryogenesis and plantlet regeneration on calluses derived from seedling explants of “Kinnow” mandarin (Citrus nobilis_Citrus deliciosa Tenore). J. Hort. Sci. 69: 231–236
Hyde CL & Phillips GC (1996) Silver nitrate promotes shoot development and plant regeneration of Chile pepper (Capsicum annuum L) via organogenesis. In Vitro Cell. Dev. Biol. Plant 32: 72–80
Jacoboni A & Standarti A (1987) Tissue culture of Jojoba (Simmondsia chinensis, Link). Acta Hortic. 212: 557–560
Mills D & Benzioni A (1992) The effect of NaCl salinity on growth and development of Jojoba clones. II. Nodal segments grown in vitro. J. Plant Physiol. 139: 737–741
Mills D, Wenkart S & Benzioni A (1997) Micropropagation of Simmondsia chinensis (Jojoba). In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 40. High-Tech and Micropropagation VI (pp 370–393). Springer-Verlag, Berlin, Heidelberg
Won Lee C & Palzkill DA (1984) Propagation of Jojoba by single node cuttings. HortScience 19: 841–842
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Roussos, P., Tolia-Marioli, A., Pontikis, C. et al. Rapid multiplication of Jojoba seedlings by in vitro culture. Plant Cell, Tissue and Organ Culture 57, 133–137 (1999). https://doi.org/10.1023/A:1006316732621
Issue Date:
DOI: https://doi.org/10.1023/A:1006316732621