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Plant Growth Regulation

, Volume 31, Issue 3, pp 147–153 | Cite as

Benzyladenine-induced adventitious shoot regeneration from hypocotyls of adzukibean (Vigna angularis {Willd.} Ohwi & Ohashi)

  • Renato A. Acenido
  • Kazumi Hattori
Article

Abstract

A complete in vitro plant regeneration systemvia adventitious shoot-bud formation from seedlinghypocotyls of adzukibean was developed. Cotyledonarynode (CN) and root explants from 4-d-old invitro-germinated seedlings of 4 cultivars werecultured on agar-solidified R medium consisting of MSsalts, B5 vitamins, 3.0% (w/v) sucrose and 4.4 µM6-benzyladenine (BA). Shoot buds arose adventitiouslyat the basipetal cut of the hypocotyl in 40 to 85%of type 1 CN (8–10 mm) explants 6 weeks later with cvsBansei aki azuki and Tanbadainagon azuki exhibitingsignificantly higher response. Shorter type 2 (4–5 mm) CN explants responded poorly (0–6%) in culturewhile intact roots were regenerative but at lowerfrequencies (5 to 31%). In cv Bansei aki azuki,optimum shoot initiation (78.7%) was achieved whentype 1 CN explants from BA-preconditioned seedlingswere re-cultured in R medium (SC0). BA supplied at8.8 µM during SC0 significantly reduced the shootinitiation response to 46.7%. In the same manner,substituting equimolar concentration (4.4 µM) ofkinetin (KN), zeatin (Z) or thidiazuron (TDZ) to BAduring SC0 yielded no or significantly lowerresponses. Mean shoot production ranged from 6.2 to9.8 shoots per explant 6 weeks after subculture (SC1)and varied significantly among the 4 cultivars used. Efficient rooting (93–96%) was achieved on excisedshoots transferred to MS salts + B5 vitamins basalmedium alone. The tissue culture system covered only16 weeks to completion. Regenerated plantlets weretransferred to soil with 86–100% survival rate andall surviving plants were morphologically normal andseed-fertile.

BA-preconditioning cotyledonary node explant cytokinin plant regeneration system tissue culture Vigna angularis 

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References

  1. 1.
    Ahroni A, Zuker A, Rozen Y, Sheijtman H and Vainstein A (1997) An efficient method for adventitious shoot regeneration from stem-segment explants of gypsophila. Plant Cell Tissue Org Cult 49: 101-106Google Scholar
  2. 2.
    Akasaki R, Hoffman-Tsay SS and Hoffman F (1998) Selection of a super-growing root culture that permits controlled switching between root cloning and direct embryogenesis. Theor Appl Genet 96: 758-764Google Scholar
  3. 3.
    Avenido RA and Hautea DM (1990) In vitro organogenesis and flowering in mungbean (Vigna radiata L. Wilczek). Philipp J Crop Sci 15: 169-173Google Scholar
  4. 4.
    Badzian T and Rybczynski JJ (1994) Cytokinin control of shoot regeneration in root segment culture of Lotus corniculatus seedling. Acta Physiol Plant 16: 61-67Google Scholar
  5. 5.
    Beattie LD and Garrett RG (1995) Adventitious shoot production from immature embryos of white clover. Plant Cell Tissue Org Cult 42: 67-72Google Scholar
  6. 6.
    Dan Y and Reichert NA (1998) Organogenic regeneration of soybean from hypocotyl explants. In Vitro Cell Dev Biol 34: 14-21Google Scholar
  7. 7.
    Daimon H and Mii M (1991) Multiple shoot formation and plantlet regeneration from cotyledonary node in peanut (Arachis hypogea L.). Japan J Breed 41: 461-466Google Scholar
  8. 8.
    Eapen S and Gill R (1986) Regeneration of plants from cultured root explants of mothbean (Vigna aconitifolia Jacq. Marechal). Theor Appl Genet 72: 384-387Google Scholar
  9. 9.
    Gamborg OL, Miller RA and Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cell. Exp Cell Res 50: 151-158Google Scholar
  10. 10.
    Ge K, Wang Y, Yuan X, Huang P, Yang J, Nie Z, Testa D and Lee N (1989) Plantlet regeneration from protoplast isolated from mesophyll cells of adzukibean (Phaseolus angularis Wight). Plant Sci 63: 209-216Google Scholar
  11. 11.
    George L and Eapen S (1994) Organogenesis and embryogenesis from diverse explants in pigeonpea (Cajanus cajan L.). Plant Cell Rep 13: 417-420Google Scholar
  12. 12.
    Heath LC, Chin SF, Spencer D and Higgins TJV (1993) In vitro regeneration of commercial cultivars of subterranean clover. Plant Cell Tissue Org Cult 35: 43-48Google Scholar
  13. 13.
    Iordan-Costache M, Lowe KC, Davey MR and Power JB (1995) Improved micropropagation of Populus spp. by Pluronic F-68. Plant Growth Reg 17: 233-239Google Scholar
  14. 14.
    Ishimoto M, Sato T, Chrispeels MJ and Kitamura K (1996) Bruchid resistance of transgenic azukibean expressing α-amylase inhibitor of common bean. Entom Exp Appl 79: 309-315Google Scholar
  15. 15.
    Kaneda Y, Tabei Y, Nishimura S, Harada K, Akihama T and Kitamura K (1997) Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybeans (Glycine max L.Merr.). Plant Cell Rep 17: 8-12Google Scholar
  16. 16.
    Khatun A, Laouar L, Davey MR, Power JB, Mulligan BJ and Lowe KC (1993) Effects of Pluronic F-68 on shoot regeneration from cultured jute cotyledons and on growth of transformed roots. Plant Cell Tissue Org Cult 34: 133-140Google Scholar
  17. 17.
    Li Z, Jarret RL, Pittman RN, Demski JW (1994) Shoot organogenesis from cultured seed explants of peanut (Arachis hypogea L.) using thidiazuron. In Vitro Cell Dev Biol 30: 187-191Google Scholar
  18. 18.
    Murashige T and Skoog S (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473-497Google Scholar
  19. 19.
    Narciso JO, Futsuhara Y, Hattori K and Wada T (1997) Effect of seed preconditioning and culture under different light intensities on callus induction and regeneration in excised cotyledon cultures of mungbean (Vigna radiata L. Wilczek). Japan J Crop Sci 66: 67-75Google Scholar
  20. 20.
    Nugent G, Wardley-Richardson T and Lu C (1991) Plant regeneration from stem and petal of carnation (Dianthus caryophyllus L.). Plant Cell Rep 10: 477-480Google Scholar
  21. 21.
    Ozaki K (1986) Plantlet formation from calli of primary leaf of adzukibean (Vigna angularis). Japan J Breed 36: 416-419Google Scholar
  22. 22.
    Santalla M, Power JB and Davey MR (1998) Efficient in vitro shoot regeneration responses of Phaseolus vulgaris and P. coccineus. Euphytica 102: 195-202Google Scholar
  23. 23.
    Sato T and Matsukawa I (1993) Agrobacterium-mediated transformation of adzukibean (Vigna angularis Ohwi and Ohashi). 2nd Plant Tissue Culture Colloqium Abstracts (pp 124-125)Google Scholar
  24. 24.
    Sato T, Asaka D, Harada T and Matsukawa I (1993) Plant regeneration from protoplasts of adzukibean (Vigna angularis Ohwi and Ohashi). Japan J Breed 43: 183-191Google Scholar
  25. 25.
    Shiva Prakash N, Pental D and Bhalla-Sarin N (1994) Regeneration of pigeonpea (Cajanus cajan) from cotyledonary node via multiple shoot formation. Plant Cell Rep 13: 623-627Google Scholar
  26. 26.
    Sujatha M and Mukta N (1996) Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell Tissue Org Cult 44: 135-141Google Scholar
  27. 27.
    Takahashi W, Matsushita J, Kobayashi T, Tanaka O and Beppu T (1998) Plant regeneration from epicotyl segment and callus of Vigna angularis (cv Tanbadainagon). Japan J Crop Sci 67: 561-567Google Scholar
  28. 28.
    Thome GCH, Santarem ER and Ferreira AG (1995) Adventitious bud induction and plant regeneration from soybean cotyledonary nodes. Int J Expl Bot 57: 127-135Google Scholar
  29. 29.
    Wright MS, Koehler SM, Hinchee MA and Carnes MG (1986) Plant regeneration by organogenesis in Glycine max. Plant Cell Rep 5: 150-154Google Scholar
  30. 30.
    Yamaguchi H (1992) Wild and weed azuki beans in Japan. Econ Bot 46: 384-394Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  1. 1.Laboratory of Plant Genetics and Breeding, Graduate School of Bioagricultural SciencesNagoya University, ChikusaNagoyaJapan

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