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Plant Cell, Tissue and Organ Culture

, Volume 56, Issue 2, pp 79–83 | Cite as

The effect of growth regulators on shoot propagation and rooting of common lavender (Lavandula vera DC)

  • L.B. Andrade
  • S. Echeverrigaray
  • F. Fracaro
  • G.F. Pauletti
  • L. Rota
Article

Abstract

Nodal segments from micropropagated plants were used to evaluate the effect of growth regulators on the in vitro shoot proliferation and rooting of Lavandula vera DC. The highest multiplication rate was obtained using MS medium supplemented with 1.0 mg l-1 of TDZ (2.25 μM) or BA (2 μM). Hyperhydricity occurred at high concentrations of these growth regulators. Rooting of the plantlets was obtained in all the media evaluated. However, rooting rates and root growth increased with increased concentrations of NAA and the reduction of the salt strength of the media. The plantlets were successfully transferred to soil and grown to maturity, exhibiting a normal development, with high uniformity and no evidences of somaclonal variation.

axillary bud breaking aromatic plant in vitro propagation Lavandula angustifolia nodal explants 

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References

  1. Bajaj YPS (1986) Biotechnology of tree improvement for rapid propagation and biomass energy production. In: YPS Bajaj (ed) Biotechnology in Agriculture and Forestry, Vol. 1. Trees I (pp 1-23). Springer Berlin Heidelberg New YorkGoogle Scholar
  2. Calvo MC & Segura J (1989) In vitro propagation of lavender. HortSci. 24:375-376Google Scholar
  3. Debergh P, Aitken-Christie J, Cohen D, Grout B, Von Arnold S, Zimmerman R & Ziv M (1992) Reconsideration of the term 'vitrification' as used in micropropagation. Plant Cell Tiss. Org. Cult. 30: 135-140CrossRefGoogle Scholar
  4. Murashige T & Skoog F (1962) A revised medium for rapid growth and biossay with tobacco tissue culture. Physiol. Plant. 15: 473-497CrossRefGoogle Scholar
  5. Moutet L (1980) Lavandin Abrialis, lavandin Grosso: what is their future? Perfumer and Flavorist 4: 27-29Google Scholar
  6. Németh G (1986) Induction of rooting. In: YPS Bajaj (ed) Biotechnology in Agriculture and Forestry, Vol. 1. Trees I (pp 49-64). Springer, Berlin, Heidelberg, New YorkGoogle Scholar
  7. Nobre J (1996) in vitro cloning and micropropagation of Lavandula stroechas from field-grown plants. Plant Cell Tiss. Org. Cult. 46: 151-155CrossRefGoogle Scholar
  8. Panizza M & Tognoni F (1991) Micropropagation of Lavandin (Lavandula officinalis Chaix _ Lavandula latifolia Villars cv. Grosso). In: YPS Bajaj (ed) Biotechnology in Agriculture and Forestry, Vol. 19. High-tech and micropropagation III (pp 295-305). Springer, Berlin, Heidelberg, New YorkGoogle Scholar
  9. Quazi MH (1980) In vitro multiplication of Lavandula spp. Ann. Bot. 45: 361-362Google Scholar
  10. Sánchez-Gras MC & Calvo MC (1996) Micropropagation of Lavandula latifolia through nodal bud culture of mature plants. Plant Cell Tiss. Org. Cult. 45: 259-261CrossRefGoogle Scholar
  11. Segura J & Calvo MC (1991) Lavandula spp. (lavender): in vitro culture, regeneration of plants, and the formation of essential oils and pigments. In: YPS Bajaj (ed) Biotechnology in Agriculture and Forestry, Vol. 15. Medicinal and Aromatic Plants III (pp 283-310). Springer, Berlin, Heidelberg, New YorkGoogle Scholar
  12. Van Nieuwkerk JP, Zimmerman R & Fordham I (1986) Thidiazuron stimulation of apple shoot proliferation in vitro. Hort Science 21: 516-518Google Scholar
  13. Weatherhead MA, Burdon J & Henshaw GG (1979) Effects of activated charcoal as an additive plant tissue culture media. Z Pflanzenphysiol 94: 399-405Google Scholar
  14. Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: PC Debergh & RH Zimmerman (eds) Micropropagation-Technology and Application (pp 45-69). Kluwer Academic Publish, DordrechtGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • L.B. Andrade
    • 1
  • S. Echeverrigaray
    • 1
  • F. Fracaro
    • 1
  • G.F. Pauletti
    • 1
  • L. Rota
    • 1
  1. 1.Institute of BiotechnologyUniversity of Caxias do SulCaxias do Sul, RSBrazil

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