Plant Cell, Tissue and Organ Culture

, Volume 55, Issue 2, pp 119–123 | Cite as

Pigment recovery from encapsulated-dehydrated Vaccinium pahalae (ohelo) cryopreserved cells

  • Rida A. Shibli
  • M.A.L. Smith
  • M.A. Shatnawi


Pigment producing in vitro cells of Vaccinium pahalae (ohelo) were tested for their ability to survive cryopreservation and retain pigment-production capacity after encapsulation-dehydration. Preculture of cells for 6 to 8 days in a medium containing 1.0 M sucrose was essential before dehydration. Reduction of bead water content before quenching in liquid nitrogen was highly correlated (r = 0.94) with increased survival rate in cells after cryopreservation. Dehydration of beads for 4 h was satisfactory for survival of cells. After quenching in liquid nitrogen, colored cells became pale, but pigment content was recovered once cells resumed growth. After three subcultures, cells regained their maximum capacity for pigment accumulation. The percentage of colored-to-total cell volume was not influenced by cryopreservation. Encapsulation-dehydration and cryopreservation did not diminish the capacity of cells to produce anthocyanins and other flavonoids.

anthocyanins cell culture cryopreservation in vitro pigment Vaccinium 


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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Rida A. Shibli
    • 1
  • M.A.L. Smith
    • 2
  • M.A. Shatnawi
    • 3
  1. 1.Biotechnology CenterJordan University of Science and TechnologyIrbidJordan
  2. 2.Department of Natural Resources and Environmental SciencesUniversity of IllinoisUrbana
  3. 3.Biotechnology CenterJordan University of Science and TechnologyIrbidJordan

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