Plant Molecular Biology

, Volume 40, Issue 4, pp 711–717 | Cite as

A mini binary vector series for plant transformation

  • Chengbin Xiang
  • Peng Han
  • Isabelle Lutziger
  • Kan Wang
  • David J. Oliver

Abstract

A streamlined mini binary vector was constructed that is less than 1/2 the size of the pBIN19 backbone (3.5 kb). This was accomplished by eliminating over 5 kb of non-T-DNA sequences from the pBIN19 vector. The vector still retains all the essential elements required for a binary vector. These include a RK2 replication origin, the nptIII gene conferring kanamycin resistance in bacteria, both the right and left T-DNA borders, and a multiple cloning site (MCS) in between the T-DNA borders to facilitate cloning. Due to the reduced size, more unique restriction sites are available in the MCS, thus allowing more versatile cloning. Since the traF region was not included, it is not possible to mobilize this binary vector into Agrobacterium by triparental mating. This problem can be easily resolved by direct transformation. The mini binary vector has been demonstrated to successfully transform Arabidopsis plants. Based on this mini binary vector, a series of binary vectors were constructed for plant transformation.

Agrobacterium tumefaciens Arabidopsis thaliana binary vector T-DNA transformation 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Chengbin Xiang
    • 1
  • Peng Han
    • 1
  • Isabelle Lutziger
    • 1
  • Kan Wang
    • 2
  • David J. Oliver
    • 1
  1. 1.Department of BotanyIowa State UniversityAmesUSA
  2. 2.Department of Plant Transformation FacilityIowa State UniversityAmesUSA

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