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Plant Molecular Biology

, Volume 40, Issue 2, pp 267–278 | Cite as

Diverse range of gene activity during Arabidopsis thaliana leaf senescence includes pathogen-independent induction of defense-related genes

  • Betania F. Quirino
  • Jennifer Normanly
  • Richard M. Amasino
Article

Abstract

To determine the range of gene activities associated with leaf senescence, we have identified genes that show preferential transcript accumulation during this developmental stage. The mRNA levels of a diverse array of gene products increases during leaf senescence, including a protease, a ribosomal protein, two cinnamyl alcohol dehydrogenases, a nitrilase and glyoxalase II. Two of the genes identified are known to be pathogen-induced. The senescence specificity of each gene was determined by characterization of transcript accumulation during leaf development and in different tissues. The increased expression of nitrilase in senescent leaves is paralleled by an increase in free indole-3-acetic acid (IAA) levels. Additionally, we have demonstrated that the induction of defense-related genes during leaf senescence is pathogen-independent and that salicylic acid accumulation is not essential for this induction. Our data indicate that the induction of certain genes involved in plant defense responses is a component of the leaf senescence program.

defense gene expression leaf senescence nitrilase pathogen-free salicylic acid 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Betania F. Quirino
    • 1
  • Jennifer Normanly
    • 2
  • Richard M. Amasino
    • 1
  1. 1.Department of BiochemistryUniversity of WisconsinMadisonUSA
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of MassachusettsAmherstUSA

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