Breast Cancer Research and Treatment

, Volume 55, Issue 1, pp 85–89 | Cite as

Mutation analysis of the putative tumor suppression gene PTEN/MMAC1 in sporadic breast cancer

  • Shou‐Tung Chen
  • Shi‐Yau Yu
  • Matty Tsai
  • Kun‐Tu Yeh
  • Jyh‐Chwan Wang
  • Min‐Chih Kao
  • Mu‐Ching Shih
  • Jan‐Gowth Chang


PTEN/MMAC1, a potential human tumor suppressor gene, has been found to have inactivating mutations in several types of cancer, including breast cancer. The incidence of breast cancer in Chinese is quite low in comparison with Caucasians, and genetic factors may play some roles. To further determine the role of PTEN/MMAC1 in breast cancer in Chinese, we used loss of heterozygosity (LOH), single strand conformation polymorphism (SSCP) with direct sequencing of variant bands, and Southern blot analysis methods to analyze mutations in PTEN/MMAC1 in 52 cases of breast cancer. None had LOH at chromosome 10q23.3. One mutation was identified, a somatic 3‐base deletion, in one case. Our results suggest PTEN/MMAC1 does not play a major role in the development of sporadic breast cancer.

Chinese mutation analysis PTEN/MMAC1 sporadic breast cancer 


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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Shou‐Tung Chen
    • 1
  • Shi‐Yau Yu
    • 2
  • Matty Tsai
    • 1
  • Kun‐Tu Yeh
    • 3
  • Jyh‐Chwan Wang
    • 1
  • Min‐Chih Kao
    • 1
  • Mu‐Ching Shih
    • 4
  • Jan‐Gowth Chang
    • 1
  1. 1.Division of Molecular Medicine, Department of Medical ResearchChina Medical College HospitalTaichungTaiwan
  2. 2.Department of SurgeryChang‐Hwa Christian HospitalChang‐HwaTaiwan
  3. 3.Department of PathologyChang‐Hwa Christian HospitalChang‐HwaTaiwan
  4. 4.Department of Laboratory MedicineChang‐Hwa Christian HospitalChang‐HwaTaiwan

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