Systematic Parasitology

, Volume 41, Issue 2, pp 105–113 | Cite as

Steinernema siamkayai n. sp. (Rhabditida: Steinernematidae), an entomopathogenic nematode from Thailand

  • S. P. Stock


Steinernema siamkayai n. sp. (Rhabditida: Steinernematidae) is a new entomopathogenic nematode isolated in Lohmsak, Thailand. Morphology, hybridisation and molecular studies indicated the distinctness of S. siamkayai n. sp. from other Steinernema spp. Diagnostic characters include: the total body length (398–495 μm) and tail length (31-41μm) of the third-stage infective juvenile and lateral field pattern with 6-8 longitudinal ridges; the presence of a tail mucro in both first and second generation females and males; the size and shape of the spicules and gubernaculum, and the arrangement of the genital papillae of the first and second generation males; and the shape of the vulva and tail of the first generation female and second generation female


Body Length Molecular Study Diagnostic Character Tail Length Field Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Amarasinghe, L.D. Hominick, W.P., Briscoe, B.R. & Reid, A.P. (1994) Occurrence and distribution of entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) in Sri Lanka. Journal of Helminthology, 68, 277–286.Google Scholar
  2. Bedding, R.A. & Akhurst, R.J. (1975) A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica, 21, 109–110.Google Scholar
  3. Courtney, W.D., Polley, D. & Miller, V.L. (1955) TAF, an improved fixative in nematode techniques. Plant Disease Report, 39, 570–571.Google Scholar
  4. Choo, H.Y. Kaya H.K. & Stock, S.P. (1995) Isolation of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Korea. Japanese Journal of Nematology, 25, 45–51.Google Scholar
  5. De Doucet, M.M.A. (1988) A new species of Neoaplectana Steiner, 1929 (Nematoda: Steinernematidae) from Cordoba, Argentina. Revue de Nemtatologie, 9, 317–323Google Scholar
  6. Gaugler, R. & Kaya, H. K. (1990) Entomopathogenic nematodes in biological control. Boca Raton, Florida: CRC Press, 365 pp.Google Scholar
  7. Jian, H., Reid, A.P. & Hunt, D.J. (1997) Steinernema ceratophorum n. sp. (Nematoda: Steinernematidae) form north-east China. Systematic Parasitology, 37, 115–125.Google Scholar
  8. Kaya, H.K. & Stock, S.P. (1997) Techniques in insect nematology. In: Lacey, L.A. (Ed.) Techniques in insect pathology. London: Academic Press, pp. 281–324.Google Scholar
  9. Maniatis, T., Fritsch, E.F. & Sambrook, J. (1989) Molecular cloning. A laboratory manual. (2nd Edn). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, p. 6.15.Google Scholar
  10. Mracek, Z. & Webster, J.M. (1993) Survey of Heterorhabditidae and Steinernematidae (Rhabditida, Nematoda) in Western Canada. Journal of Nematology, 25, 710–717.Google Scholar
  11. Poinar, G.O., Jr (1990) Taxonomy and biology of Steinernematidae and Heterorhabditidae. In: Gaugler, R. & Kaya, H.K. (Eds.) Entomopathogenic nematodes in biological control. Boca Raton, Florida: CRC Press, pp. 23–61.Google Scholar
  12. Reid, A.P. & Hominick, W.P. (1993) Cloning of rDNA repeat unit from a British entomopathogenic nematode (Steinernematidae) and its potential for species identification. Parasitology, 107, 529–536Google Scholar
  13. Roman, J. & Figueroa, W. (1994) Steinernema puertoricensis n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Puerto Rico. Journal of Agriculture of the University of Puerto Rico 78, 167–175.Google Scholar
  14. Rosa, J.S., Martins, A., Mendes, C., Amaral, J.J., Lacey, L.A. & Simoes, N. (1994) Natural occurrence of soil entomopathogens in the Azores Islands. Abstracts. VIth International Colloquium on Invertebrate Pathology and Microbial Control, 2, 275–276.Google Scholar
  15. Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4, 67–69.Google Scholar
  16. Stock, S.P. (1995). Isolation of entomopathogenic nematodes from the Pampean region of Argentina. Nematropica, 25, 143–148Google Scholar
  17. Stock, S.P., Choo, H.Y. & Kaya, H.K. (1997) An entomopathogenic nematode, Steinernema monticolum (Rhabditida: Steinernematidae) from Korea with a key to other species. Nematologica, 43, 15–29.Google Scholar
  18. Vrain, T.C., Wakarchuk, D.A., Levesque, A.C. & Hamilton, R.I. (1992) Intraspecific rDNA restriction fragment length polymorphisms in the Xiphinema americanum group. Fundamental and Applied Nematology, 15, 563–574.Google Scholar
  19. White, G.F. (1927) A method for obtaining infective nematode larvae from cultures. Science, 66, 302–303.Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • S. P. Stock

There are no affiliations available

Personalised recommendations