Plant Molecular Biology

, Volume 38, Issue 6, pp 1169–1178 | Cite as

Expression of an anther-specific chalcone synthase-like gene is correlated with uninucleate microspore development in Nicotiana sylvestris

  • Ivan Atanassov
  • Eugenia Russinova
  • Ludmil Antonov
  • Atanas Atanassov

Abstract

Two cDNA clones, specifically expressed in Nicotiana sylvestris anthers during uninucleate microspore development, were isolated using a subtractive hybridization approach. Sequence analysis showed that one of them, NSCHSLK, displayed a high level of similarity to several anther-specific chalcone synthase-like (CHSLK) proteins and an ORF from chromosome 1 of Arabidopsis thaliana. A lower, but significant, similarity to chalcone synthases and closely related enzymes (CHSRE) was also detected. The structure of the nschslk gene was found to be typical of the chalcone (chs) / stilbene (sts) synthase family. Expression of NSCHSLK mRNA was confined to microspores and tapetal cells. UV-irradiation or infection with Phytophthora parasitica var. nicotianae of transgenic Nicotiana benthamiana plants carrying a chimeric nschslk/GUS gene indicated that the nschslk promoter exhibits the same anther-specific, developmentally regulated expression pattern. Comparison of CHSRE and CHSLK polypeptide sequences revealed some important similarities and differences between the two groups. The data presented in this study, suggest that the anther-specific chslk genes represent a separate sub-family of plant polyketide synthases related to chs/sts in terms of gene structure, polypeptide sequence and the possible catalytic mechanism, but differing in substrate/product specificity. The putative role of CHSLK enzymes in anther development and particularly in exine synthesis is discussed.

anther Arabidopsis thaliana chalcone synthase-like microspore Nicotiana sylvestris tapetum 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Ivan Atanassov
    • 1
  • Eugenia Russinova
    • 1
  • Ludmil Antonov
    • 1
  • Atanas Atanassov
    • 2
  1. 1.Intsitute of Genetic EngineeringKostinbrod-2Bulgaria
  2. 2.De Montfort University, Norman Borlaug Center for Plant Science ResearchKostin-brod-2Bulgaria

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