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Plant Molecular Biology

, Volume 37, Issue 5, pp 763–772 | Cite as

Conserved structure and function of the Arabidopsis flowering time gene CONSTANS in Brassica napus

  • Laurian S. Robert
  • Frances Robson
  • Andrew Sharpe
  • Derek Lydiate
  • George Coupland
Article

Abstract

The Arabidopsis thaliana CONSTANS (CO) gene which promotes flowering in long days was recently isolated by chromosome walking. The mapping of QTLs controlling flowering time in Brassica species has identified genomic regions that contain homologues of the CO gene. Four genes homologous to the Arabidopsis CO gene were isolated from a pair of homoeologous loci in each of two doubled-haploid Brassica napus lines displaying different flowering times, N-o-1 and N-o-9. The four genes, BnCOa1, BnCOa9, BnCOb1 and BnCOb9, are located on linkage groups N10 and N19, and are highly similar to each other and to the Arabidopsis CO gene. Two regions of the proteins are particularly well conserved, a N-terminal region with two putative zinc fingers and a C-terminal region which may contain a nuclear localization signal. All four genes appear to be expressed in B. napus. The BnCOa1 allele was shown to complement the co-2 mutation in Arabidopsis in a dosage-dependent manner causing earlier flowering than in wild type under both long- and short-day conditions.

Arabidopsis thaliana Brassica napus constans flowering zinc finger 

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References

  1. 1.
    Albani D, Robert LS: Cloning and characterization of a Brassica napus gene encoding a homologue of the B subunit of a heteromeric CCAAT-binding factor. Gene 167: 209–213 (1995).CrossRefPubMedGoogle Scholar
  2. 2.
    Amasino RM: Control of flowering time in plants. Curr Opin Genet Devel 6: 480–487 (1996).Google Scholar
  3. 3.
    Coupland G: Genetic and environmental control of flowering time in Arabidopsis. Trends Genet 11: 393–397 (1995).PubMedGoogle Scholar
  4. 4.
    Jones JDG, Shlumukov L, Carland F, English J, Scofield SR, Bishop GJ, Harrison K: Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgen Res 1: 285–297 (1992).Google Scholar
  5. 5.
    Joshi CP: An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucl Acids Res 15: 6643–6653 (1987).PubMedGoogle Scholar
  6. 6.
    Keith DJ: Genetic analysis of quantitative traits in Brassica napus. PhD thesis, University of Anglia, Norwich, UK (1995).Google Scholar
  7. 7.
    Kisu Y, Esaka m, Suzuki M: Putative zinc-binding domain of plant transcription factor, AOBP, is related to DNA-binding domains of steroid hormone receptors and GATA1. Proc Jpn Acad 71: 288–292 (1995).Google Scholar
  8. 8.
    Koornneef M, Hanhart CJ, van der Veen JH: A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana. Mol Gen Genet 229: 57–66 (1991).PubMedGoogle Scholar
  9. 9.
    Lagercrantz U, Putterill J, Coupland G, Lydiate D: Comparative mapping in Arabidopsis and Brassica, fine scale genome collinearity and congruence of genes controlling flowering time. Plant J 9: 13–20 (1996).PubMedGoogle Scholar
  10. 10.
    Lee IL, Aukerman MJ, Gore SL, Lohman KN, Michaels SD, Weaver LM, John MC, Feldmann KA, Amasimo RM: Isolation of LUMINIDEPENDENS: a gene involved in the control of flowering time in Arabidopsis. Plant Cell 6: 75–83 (1994).CrossRefPubMedGoogle Scholar
  11. 11.
    Lippuner V, Cyert MS, Gasser CS: Two classes of plant cDNA clones differentially complement yeast calcineurin mutants and increase salt tolerance of wild-type yeast. J Biol Chem 271: 12859–12866 (1996).PubMedGoogle Scholar
  12. 12.
    Logemann J, Schell J, Willmitzer L: Improved method for the isolation of RNA from plant tissues. Anal Biochem 163: 16–20 (1987).PubMedGoogle Scholar
  13. 13.
    Macknight R, Bancroft I, Page T, Lister C, Schmidt R, Love K, Westphal L, Murphy G, Sherson S, Cobbett C, Dean C: FCA, a gene controlling flowering time in Arabidopsis, encodes a protein containing RNA-binding domains. Cell 89: 737–745 (1997).PubMedGoogle Scholar
  14. 14.
    Omichinski JG, Clore GM, Schaad O, Felsenfeld G, Trainor C, Appella E, Stahl SJ, Gronenborn AM: NMR structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1. Science 261: 438–446 (1993).PubMedGoogle Scholar
  15. 15.
    Ouellet T, Rutledge RG, Miki BL: Members of the acetohydroxyacid synthase multigene family of Brassica napus have divergent patterns of expression. Plant J 2: 321–330 (1992).CrossRefPubMedGoogle Scholar
  16. 16.
    Parkin IAP, Sharpe AG, Keith DJ, Lydiate DJ: Identification of the A and C genomes of amphidiploid Brassica napus (oilseed rape). Genome 38: 1122–1131 (1995)Google Scholar
  17. 17.
    Putterill J, Robson F, Lee K, Simon R, Coupland G: The constans gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80: 847–857 (1995).PubMedGoogle Scholar
  18. 18.
    Putterill JJ, Ledger S, Lee K, Robson F, Murphy g, Coupland G: The flowering-time gene CONSTANS and homologue CONSTANS LIKE 1 (Accession No. Y10555 and Y10556) exist as a tandem repeat on chromosome 5 of Arabidopsis. Plant Physiol 114: 396 (1997).Google Scholar
  19. 19.
    Robert LS, Gerster J, Allard S, Cass L, Simmonds J: Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum. Plant J 6: 927–933 (1994).CrossRefPubMedGoogle Scholar
  20. 20.
    Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).Google Scholar
  21. 21.
    Schmiedeskamp M, Klevit RE: Zinc finger diversity. Curr Opin Struct Biol 4: 28–35 (1994).Google Scholar
  22. 22.
    Sharpe AG, Parkin IAP, Keith DJ, Lydiate DJ: Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). Genome 38: 1112–1121 (1995).Google Scholar
  23. 23.
    Simon R, Coupland G: Arabidopsis genes that regulate flowering time in response to day-length. Cell Devel Biol 7: 419–425 (1996).Google Scholar
  24. 24.
    Simon R, Igeňo MI, Coupland G: Activation of floral meristem identity genes in Arabidopsis. Nature 384: 59–62 (1997).Google Scholar
  25. 25.
    Valvekens D, Van Montagu M, Van Lijsebettens M: Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci USA 87: 5536–5540 (1988)Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Laurian S. Robert
    • 1
  • Frances Robson
    • 2
  • Andrew Sharpe
    • 3
  • Derek Lydiate
    • 3
  • George Coupland
    • 2
  1. 1.Eastern Cereal and Oilseed Research Centre, Central Experimental FarmOttawaCanada
  2. 2.Molecular Genetics Department, John Innes Centre, Colney LaneNorwichUK
  3. 3.Brassica and Oilseeds Research Department, John Innes Centre, Colney LaneNorwichUK

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