Plant Molecular Biology

, Volume 38, Issue 6, pp 977–982 | Cite as

Differential regulation of genes encoding 1-aminocyclopropane- 1-carboxylate (ACC) synthase in etiolated pea seedlings: effects of indole-3-acetic acid, wounding, and ethylene

  • Scott C. Peck
  • Hans Kende
Article

Abstract

Treatment of 5- to 6-day-old etiolated pea (Pisum sativum L.) seedlings with indole-3-acetic acid (IAA) induced within 15 min an increase in the transcript levels of two genes encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase, Ps-ACS1 and Ps-ACS2. Simultaneous treatment with ethylene inhibited this increase and also caused a decrease in ACC synthase enzyme activity as compared to that of seedlings treated with IAA alone. These results indicate that ethylene inhibits its own biosynthesis by decreasing ACC synthase transcript levels via a negative feedback loop. Wounding of pea stems had no effect on the expression of Ps-ACS1, but led within 10 min to an increase in the mRNA levels of Ps-ACS2. This increase was also inhibited by ethylene. The wound signal was transmitted over a distance of at least 4 cm through the stem with no delay in induction or response intensity. The rapid transmission of the wound response is consistent with the possibility that a hydraulic or electric signal is responsible for the spread of the wound response.

1-aminocyclopropane-1-carboxylate (ACC) synthase auxin wounding ethylene Pisum sativum 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Abeles FB, Morgan PW, Saltveit Jr ME: Ethylene in Plant Biology. Academic Press, New York (1992).Google Scholar
  2. 2.
    Bögre L, Ligterink W, Meskiene I, Barker PJ, Heberle-Bors E, Huskisson NS, Hirt H: Wounding induces the rapid and transient activation of a specific MAP kinase pathway. Plant Cell 9: 75–83 (1997).Google Scholar
  3. 3.
    Boller T, Herner RC, Kende H: Assay for and enzymatic formation of an ethylene precursor, 1-aminocyclopropane-1-carboxylic acid. Planta 145: 293–303 (1979).Google Scholar
  4. 4.
    Botella JR, Arteca JM, Schlagnhaufer CD, Arteca RN, Phillips AT: Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid. Plant Mol Biol 20: 425–436 (1992).Google Scholar
  5. 5.
    Burg SP, Burg EA: The interaction between auxin and ethylene and its role in plant growth. Proc Natl Acad Sci USA 55: 262–269 (1966).Google Scholar
  6. 6.
    Jones JF, Kende H: Auxin-induced ethylene biosynthesis in subapical stem sections of etiolated seedlings of Pisum sativum L. Planta 146: 649–656 (1979).Google Scholar
  7. 7.
    Kang BG, Yocum CS, Burg SP, Ray PM: Ethylene and carbon dioxide, mediation of hypocotyl hook-opening response. Science 156: 958–959 (1967)Google Scholar
  8. 8.
    Kende H: Ethylene biosynthesis. Annu Rev Plant Physiol Plant Mol Biol 44: 283–307 (1993).Google Scholar
  9. 9.
    Kim WT, Silverstone A, Yip WK, Dong GD, Yang SF: Induction of 1-aminocyclopropane-1-carboxylate synthase mRNA by auxin in mung bean hypocotyls and cultured apple shoots. Plant Physiol 98: 465–471 (1992).Google Scholar
  10. 10.
    Liang X, Abel S, Keller JA, Shen NF, Theologis A: The 1-aminocyclopropane-1-carboxylate synthase gene family of Arabidopsis thaliana. Proc Natl Acad Sci USA 89: 11046–11050 (1992).Google Scholar
  11. 11.
    Nakajima N, Mori H, Yamazaki K, Imaseki H: Molecular cloning and sequence of a complementary DNA encoding 1-aminocyclopropane-1-carboxylate synthase induced by tissue wounding. Plant Cell Physiol 31: 1021–1029 (1990).Google Scholar
  12. 12.
    Olson DC, Oetiker JH, Yang SF: Analysis of LE-ACS3, a 1-aminocyclopropane-1-carboxylic acid synthase gene expressed during flooding in the roots of tomato plants. J Biol Chem 270: 14056–14061 (1995).Google Scholar
  13. 13.
    Olson DC, White JA, Edelman L, Harkins RN, Kende H: Differential expression of two genes for 1-aminocyclopropane-1carboxylate synthase in tomato fruits. Proc Natl Acad Sci USA 88: 5340–5344 (1991).Google Scholar
  14. 14.
    Peck SC, Olson DC, Kende H: A cDNA sequence encoding 1-aminocyclopropane-1-carboxylate oxidase from pea. Plant Physiol 101: 689–690 (1993).Google Scholar
  15. 15.
    Peck SC, Kende H: Sequential induction of the ethylene biosynthetic enzymes by indole-3-acetic acid in etiolated peas. Plant Mol Biol 28: 293–301 (1995).Google Scholar
  16. 16.
    Peck, SC, Kende H: A gene encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase produces two transcripts: elucidation of a conserved response. Plant J 14: 573–581 (1998).Google Scholar
  17. 17.
    Saltveit Jr ME, Dilley DR: Rapidly induced wound ethylene from excised segments of etiolated Pisum sativum L., cv. Alaska. I. Characterization of the response. Plant Physiol 61: 447–450 (1978).Google Scholar
  18. 18.
    Saltveit Jr ME, Dilley DR: Rapidly induced wound ethylene from excised segments of etiolated Pisum sativum L., cv. Alaska. II. Oxygen and temperature dependency. Plant Physiol 61: 675–679 (1978).Google Scholar
  19. 19.
    Sato T, Theologis A: Cloning the mRNA encoding 1-aminocyclopropane-1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants. Proc Natl Acad Sci USA 86: 6621–6625 (1989).Google Scholar
  20. 20.
    Stratmann JW, Ryan CA: Myelin basic protein kinase activity in tomato leaves is induced systemically by wounding and increases in response to systemin and oligosaccharide elicitors. Proc Natl Acad Sci USA 94: 11085–11089 (1997).Google Scholar
  21. 21.
    Van Der Straeten D, Van Wiemeersch L, Goodman HM, Van Montagu M: Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato. Proc Natl Acad Sci USA 87: 4859–4863 (1990).Google Scholar
  22. 22.
    Yang SF, Hoffman NE Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35: 155–189 (1984).Google Scholar
  23. 23.
    Yip W-K, Moore T, Yang SF: Differential accumulation of transcripts for four tomato 1-aminocyclopropane-1-carboxylate synthase homologs under various conditions. Proc Natl Acad Sci USA 89: 2475–2479 (1992).Google Scholar
  24. 24.
    Yu Y-B, Adams DO, Yang SF: 1-Aminocyclopropanecarboxylate synthase, a key enzyme in ethylene biosynthesis. Arch Biochem Biophys 198: 280–286 (1979).Google Scholar
  25. 25.
    Zarembinski TI, Theologis A: Anaerobiosis and plant growth hormones induce two genes encoding 1-aminocyclopropane-1-carboxylate synthase in rice (Oryza sativa L.). Mol Biol Cell 4: 363–373 (1993).Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Scott C. Peck
    • 2
  • Hans Kende
    • 1
  1. 1.MSU-DOE Plant Research LaboratoryMichigan State UniversityEast LansingUSA
  2. 2.Friedrich Miescher InstituteBaselSwitzerland

Personalised recommendations