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Plant Growth Regulation

, Volume 26, Issue 1, pp 57–61 | Cite as

Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using Thidiazuron

  • Tapan Kumar Mondal
  • Amita Bhattacharya
  • Anil Sood
  • Paramvir Singh Ahuja
Article

Abstract

The effect of thidiazuron (TDZ) on the micropropagation of Camellia sinensis (China hybrid) was compared with that of benzylaminopurine (BAP) using nodal segments from in vitro raised seedlings. Extremely low concentrations of TDZ (1pM–100nM) alone were effective in inducing shoot bud proliferation and maintaining high rates of shoot multiplication on hormone-free media. On the other hand, higher concentrations of BAP (1–10μM) and its continued presence were required to initiate and sustain shoot proliferation. While wider ranges of BAP combined favourably with auxins like NAA or IBA, only specific combinations of TDZ and NAA were effective for shoot proliferation. TDZ treated explants yielded healthy shoots, with sturdy leaves, even during the initial stages of growth, whereas, the effect of BAP was cumulative over subcultures in attaining a high proliferative rate.

axillary shoot multiplication Camellia sinensis (L.) O. Kuntze nodal segments Thidiazuron woody plant 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Tapan Kumar Mondal
    • 1
  • Amita Bhattacharya
    • 1
  • Anil Sood
    • 1
  • Paramvir Singh Ahuja
    • 1
  1. 1.Division of BiotechnologyInstitute of Himalayan Bioresource TechnologyPalampur-India Fax: +

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