Plant Growth Regulation

, Volume 26, Issue 1, pp 57–61 | Cite as

Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using Thidiazuron

  • Tapan Kumar Mondal
  • Amita Bhattacharya
  • Anil Sood
  • Paramvir Singh Ahuja


The effect of thidiazuron (TDZ) on the micropropagation of Camellia sinensis (China hybrid) was compared with that of benzylaminopurine (BAP) using nodal segments from in vitro raised seedlings. Extremely low concentrations of TDZ (1pM–100nM) alone were effective in inducing shoot bud proliferation and maintaining high rates of shoot multiplication on hormone-free media. On the other hand, higher concentrations of BAP (1–10μM) and its continued presence were required to initiate and sustain shoot proliferation. While wider ranges of BAP combined favourably with auxins like NAA or IBA, only specific combinations of TDZ and NAA were effective for shoot proliferation. TDZ treated explants yielded healthy shoots, with sturdy leaves, even during the initial stages of growth, whereas, the effect of BAP was cumulative over subcultures in attaining a high proliferative rate.

axillary shoot multiplication Camellia sinensis (L.) O. Kuntze nodal segments Thidiazuron woody plant 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Agarwal B, Singh U and Maitreyi B (1992) In vitro clonal propagation of tea (Camellia sinensis). Plant Cell Tissue Organ Cult 30: 1-5Google Scholar
  2. 2.
    Arulpragasam PV and Latiff R (1986) Studies on the tissue culture of tea (Camellia sinensis (L.) 0. Kuntze.). 1. Development of a culture method for the multiplication of shoots. Sri L J Tea Sci 55(1): 44-47Google Scholar
  3. 3.
    Bag N, Palni LMS and Nandi SK (1997) Mass propagation of tea using tissue culture methods. Physiol Mol Biol Plants 3: 99-103Google Scholar
  4. 4.
    Bohmer P, Meyer B and Jacobsen HJ (1995) Thidiazuron induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus. Plant Cell Rep 15: 26-29Google Scholar
  5. 5.
    Banerjee M and Agarwal B (1990) In vitro rooting of tea (Camellia sinensis. (L). O. Kuntze.). Ind J Exp Bot 28: 936-939Google Scholar
  6. 6.
    Capelle SC, Mok DWS, Kirchner SC and Mok SC (1983) Effects of thidiazuron on cytokinin autonomy and the metabolism on N6-(2-isopentenyl) [8-14C] adenosine in callus tissues of Phaseolus lunatus L. Plant Physiol 73: 796-802Google Scholar
  7. 7.
    Chalupa V (1987) Effect of benzylaminopurine and thidiazuron on in vitro shoot proliferation of Tilia cordata Mill., Sorbus aucuparia L. and Robinia pseudoacacia L. Biol Plant (Praha) 29: 425-429Google Scholar
  8. 8.
    Chalupa V (1988) Large scale micropropagation of Quercus robur L. using adenine type cytokinins and thidiazuron to stimulate shoot proliferation. Biol Plant (Praha) 30: 414-421Google Scholar
  9. 9.
    Dodd AW (1994) Tissue culture of tea (Camellia sinensis) (L.) O. Kuntze - A Review. Intern J Trop Agric Vol. XII 3 & 4: 212- 247Google Scholar
  10. 10.
    Huetteman A and Preece EJ (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33: 105-119Google Scholar
  11. 11.
    Jha T and Sen SK (1992) Micropropagation of an elite Darjeeling tea clone. Plant Cell Rep 11: 101-104Google Scholar
  12. 12.
    Kerns HR and Meyer MM Jr (1987) Diligence finds chemical key to micropropagating a new maple. Amer Nurseryman 165: 104-110Google Scholar
  13. 13.
    Lloyd G and McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Comb Proc Intl Pl Prop Soc 30: 421-427Google Scholar
  14. 14.
    Malik KA and Saxena PK (1992) Regeneration in Phaseolus vulgaris L. High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Plant 186: 384-389Google Scholar
  15. 15.
    McCown BH, McCabe DE, Russell DR, Robinson DJ, Barton KA and Raffa KF (1991) Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration. Plant Cell Rep 9: 590-594Google Scholar
  16. 16.
    Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y and Okamoto T (1982) Cytokinin activity of N-phenyl-N1-1,2,3-thiadiazol-5-ylurea (thidiazuron). Phytochem 21: 1509-1511Google Scholar
  17. 17.
    Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473-497Google Scholar
  18. 18.
    Murthy BNS, Victor J, Singh RPS, Fletcher RA and Saxena, PK (1996) In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron. Plant Growth Reg 19: 233-240Google Scholar
  19. 19.
    Phukan MK and Mitra GC (1984) Regeneration of tea shoots from nodal explants in tissue culture. Curr Sci 53: 874-876Google Scholar
  20. 20.
    Sankhla D, Davis TD and Sankhla N (1994) Thidiazuron-induced in vitro shoot formation from roots of intact seedlings of Albizzia julibrissin. Plant Growth Reg 14: 267-272Google Scholar
  21. 21.
    Sood A, Palni LMS, Sharma M, Rao DV, Chand G and Jain NK (1993) Micropropagation of tea using cotyledon culture and encapsulated somatic embryos. J Pl Crops 21 (Suppl.): 295-300Google Scholar
  22. 22.
    Van Nieuwkerk JP, Zimmerman RH and Fordham I (1986) Thidiazuron stimulation of apple shoot proliferation in vitro. Hort Sci 21: 516-518Google Scholar
  23. 23.
    Vieitez A (1994) Somatic embryogenesis in Camellia spp. In: Jain SM, Gupta P and Newton R (eds) Somatic enbryogenesis in woody plants. The Netherlands: Kluwer Academic PublishersGoogle Scholar
  24. 24.
    Yang G and Read PE (1991) In vitro propagation of Acanthopanax sieboldianus. Hort Sci 26: 749 (Abstr.)Google Scholar
  25. 25.
    Zhou J, Ma H, Guo F and Luo X (1994) Effect of thidiazuron on somatic embryogenesis of Cayratia japonica. Plant Cell Tissue Organ Cult 36: 73-79Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Tapan Kumar Mondal
    • 1
  • Amita Bhattacharya
    • 1
  • Anil Sood
    • 1
  • Paramvir Singh Ahuja
    • 1
  1. 1.Division of BiotechnologyInstitute of Himalayan Bioresource TechnologyPalampur-India Fax: +

Personalised recommendations