Breast Cancer Research and Treatment

, Volume 50, Issue 1, pp 11–25 | Cite as

Genomic organization of the canine p53 gene and its mutational status in canine mammary neoplasia

  • Lee Lee Chu
  • Gerard R. Rutteman
  • Julius M.C. Kong
  • Majid Ghahremani
  • Martin Schmeing
  • Wim Misdorp
  • Evert van Garderen
  • Jerry Pelletier


To determine whether canine malignancies share common genetic lesions with their human counterparts, and are thus potentially interesting model systems in which to pose questions regarding tumor etiology and progression, we have elucidated the entire exon/intron structure of the canine p53 gene. A search for p53 gene abnormalities in mammary tumor tissue was undertaken utilizing single strand conformation polymorphism analysis. Mutations were detected in exons 4, 5, 6, and 7 of the p53 gene and consisted of nonsense, splicing, and frameshift mutations. None of 11 benign tumors and 6 of 40 primary carcinomas (15%) were found to harbor subtle p53 mutations. In 14 carcinomas examined the results in primary tumors and metastases were the same. These findings implicate involvement of this gene in the genesis of some malignant canine tumors, in a fashion similar to their human counterparts.

p53 breast cancer canine mutational analysis 


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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Lee Lee Chu
    • 1
  • Gerard R. Rutteman
    • 2
  • Julius M.C. Kong
    • 1
  • Majid Ghahremani
    • 1
  • Martin Schmeing
    • 1
  • Wim Misdorp
    • 2
    • 3
  • Evert van Garderen
    • 4
  • Jerry Pelletier
    • 1
    • 5
  1. 1.Department of BiochemistryCanada
  2. 2.Department of Clinical Sciences of Companion AnimalsNetherlands
  3. 3.Department of Pathology, Faculty of Veterinary MedicineUniversity of UtrechtNetherlands
  4. 4.Department of Pathology, Faculty of Veterinary MedicineUniversity of UtrechtNetherlands
  5. 5.Department of OncologyMcGill UniversityMontréalCanada

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