Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 50, Issue 2, pp 83–89 | Cite as

High frequency shoot regeneration and plantlet formation from root tip of garlic

  • Muhammad Shahidul Haque
  • Tomikichi Wada
  • Kazumi Hattori
Article

Abstract

An efficient and novel method of direct shoot regeneration from root tips in garlic was developed. The influence of growth regulators, basal media and age of root explant on shoot initiation and proliferation was examined. The best growth regulator combination was 1-naphthaleneacetic acid and 6-benzyladenine at 1 and 10 µM, respectively, inducing shoot initiation from 75% of the explants. The frequency of shoot initiation on different basal media was similar. Explant root tips from plantlets taken 15 to 18 days after sprouting showed the highest shoot initiation (95%). In contrast to Murashige and Skoog medium, which produced more than 10 shoots per explant, B5 medium produced smaller shoots, although the number was higher. Rooting of individual shoots was induced after transfer to medium without growth regulators. Plantlets, after acclimatization in a growth cabinet, were successfully transplanted to the field, and no phenotypic variation was observed among them. The technique has potential applicability for rapid propagation of garlic.

Allium sativum L. micropropagation root tip culture shoot initiation shoot proliferation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abo EI-Nil MM (1977) Organogenesis and embryogenesis in callus cultures of Garlic (Allium sativum L.). Plant Sci Lett. 9: 259–264CrossRefGoogle Scholar
  2. Bhojwani SS (1980) In vitro propagation of garlic by shoot proliferation. Scientia Hort. 13: 47–52CrossRefGoogle Scholar
  3. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu Cl & Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. (Peking) 18: 659–668Google Scholar
  4. Dunstan DI & Short KC (1977) Improved growth of tissue cultures of the onion, Allium cepa. Physiol. Plant. 41: 70–72CrossRefGoogle Scholar
  5. Gamborg OL (1984) Plant cell cultures. In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 1 (pp 18–26). Academic Press, Inc., LondonGoogle Scholar
  6. Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158PubMedCrossRefGoogle Scholar
  7. Islam R, Hossein M, Reza MA, Mamun ANK & Joarder OI (1996) Adventitious shoot regeneration from root tips of intact seedlings of Aegle marmelos. J. Hort. Sci. 71: 995–1000Google Scholar
  8. Koch M, Tanami Z & Salomon R (1995) Improved regeneration of shoots from garlic callus. HortScience 30: 378Google Scholar
  9. Mohamed-Yasseen Y, Barringer SA & Splittstoesser WE (1995) In vitro bulb production from Allium spp. In Vitro Cell. Dev. Biol. 31: 51–52CrossRefGoogle Scholar
  10. Mohamed-Yasseen Y, Splittstoesser WE & Litz RE (1994) In vitro shoot proliferation and production of sets from garlic and shallot. Plant Cell Tiss. Org. Cult. 36: 243–247CrossRefGoogle Scholar
  11. Murashige T & Skoog F (1962) A revised medium for rapid growth and boiassays with tobacco tissue cultures Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  12. Nagakubo T, Nagasawa A & Ohkawa H (1993) Micropropagation of garlic through in vitro bulblet formation. Plan Cell Tiss. Org. Cult. 32: 175–183CrossRefGoogle Scholar
  13. Nagasawa A & Finer JJ (1988) Induction of morphogenic callus cultures from leaf tissue of garlic. HortScience 23: 1068–1070Google Scholar
  14. Novak FJ (1990) Allium tissue culture. In: Rabinowitch HD & Brewster JL (eds) Onions and Allied Crops, Vol 1 (pp 233–250). CRC, Boca Raton, FloridaGoogle Scholar
  15. Novak FJ, Havel L & Dolezel J (1982) In vitro breeding system in Allium In: Fujiwara A (ed) Plant Tissue Culture (pp 767–768). Maruzen, TokyoGoogle Scholar
  16. Pierik RLM (1987) In vitro Culture of Higher Plants. Martinus Nijhoff Publishers, DordrechtGoogle Scholar
  17. Rauber M & Grunewaldt J (1988) In vitro regeneration in Allium species. Plant Cell Rep. 7: 426–429Google Scholar
  18. Seabrook JEA (1994) In vitro propagation and bulb formation of garlic. Can J. Plant Sci. 74: 155–158Google Scholar
  19. Sharma KK & Thorpe TA (1989) In vitro regeneration of shoot buds and plantlets from seedling root segments of Brassica napus L. Plant Cell Tiss. Org. Cult. 18: 129–141CrossRefGoogle Scholar
  20. Shuto H, Abe T & Sasahara T (1993) In vitro propagation of plants from root apex-derived calli in chinese chive (Allium tuberosum ROTTLER) and garlic (Allium sativum L.). Japan J. Breed. 43: 349–354Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Muhammad Shahidul Haque
    • 1
  • Tomikichi Wada
    • 1
  • Kazumi Hattori
    • 1
  1. 1.Laboratory of Theory of Agronomy and Plant BreedingSchool of Agricultural Sciences, Nagoya University, ChikusaNagoyaJapan

Personalised recommendations